Electron staining of myocardial amyloidosis by different preparation methods and tea instead of uranium
ZHAO Ranxu1 WEI Yingjie1▲ LI Li2 WANG Qingzhi2 LU Yang2 XU Hongyu2 YOU Yiwei2
1.Department of Electron Microscopy Platform of National Key Laboratory of Cardiovascular Diseases, Chinese Academy of Medical Sciences Peking Union Medical College National Center for Cardiovascular Diseases Fuwai Hospital, Beijing 100037, China;
2.Department of Pathology, Chinese Academy of Medical Sciences Peking Union Medical College National Center for Cardiovascular Diseases Fuwai Hospital, Beijing 100037, China
Abstract：Objective To test whether oolong tea extract can be used as an electronic stain instead of uranyl acetate (UA) for myocardial amyloidosis staining. In the process of staining, different staining methods were used and improve the preparation methods and compare their safety and efficiency. Methods A total of 40 cases of clinically suspected amyloidosis were collected from Fuwai Hospital of the Chinese Academy of Medical Sciences from January to December 2017, and 10 cases of cardiac tissue positive after pathological observation and special staining. The myocardial tissues were prepared by conventional encapsulation and improvement of resin 812, and then two kinds of different stains (3% uranyl acetate solution, 0.5% oolong tea extract) were applied to the ultrathin section samples under the conditions of counterstaining lead or not counterstaining lead, and two different staining methods (drip method, slice box staining) electronic staining, and then the differences were compared under a transmission electron microscope. Results Under the condition of repeated staining of lead, the results of staining of amyloid substance in myocardium with two kinds of electron staining agents and the improved preparation method were all satisfactory. The background of the slice was clean, the contrast was high and the obtained image was clear. Conclusion In the observation of myocardial amyloidosis, oolong tea extract with section box staining and the improved preparation method can more safely and quickly replace dioxide-uranium acetate for transmission electron microscope electron staining.
赵然旭1 魏英杰1▲ 李莉2 王清峙2 陆洋2 徐红宇2 有一伟2. 超薄切片不同制备方法和茶代铀进行心肌淀粉样变性的电子染色[J]. 中国医药导报, 2019, 16(28): 17-20.
ZHAO Ranxu1 WEI Yingjie1▲ LI Li2 WANG Qingzhi2 LU Yang2 XU Hongyu2 YOU Yiwei2. Electron staining of myocardial amyloidosis by different preparation methods and tea instead of uranium. 中国医药导报, 2019, 16(28): 17-20.
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2019, Vol. 16 
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