miR-361-3p调节恶性胶质瘤细胞功能的研究

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摘要目的研究miR-361-3p在恶性胶质瘤（GB）细胞中的功能。方法培养LN299和U87MG细胞及人正常星形胶质细胞（NHA）。miR-361-3p的mimic或inhibitor及MTCP1的siRNA（si-MTCP1）转染U87MG细胞。RT-PCR和Western blot检测目标基因水平的变化。CCK-8和Caspase3试剂盒分别检测细胞的增殖和凋亡。荧光素酶基因报告实验检测miR-361-3p和MTCP1基因的结合。结果与NHA比较，miR-361-3p在LN299和U87MG中低表达。U87MG转染mimic可抑制MTCP1的表达和细胞增殖率，升高Caspase3活性，并减少WT-MTCP1的荧光素酶活性。U87MG转染inhibitor增加细胞增殖率，而转染si-MTCP1后细胞凋亡增加。结论 miR-361-3p可能通过抑制MTCP1来抑制GB细胞的增殖并促进凋亡。

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	郭小叶冒平王佳廉海平王伟白晓斌宋锦宁

关键词 ：恶性胶质瘤, 细胞增殖, 凋亡, miR-361-3p

Abstract：Objective To study the role of miR-361-3p in glioblastoma (GB) cells. Methods LN299, U87MG and normal human astrocytes (NHA) were cultured. miR-361-3p mimic or inhibitor and MTCP1 siRNA (si-MTCP1) were transfected into U87MG cells, respectively. Gene expression was tested by RT-PCR and Western blot. Cell proliferation and apoptosis were measured by CCK-8 and Caspase3 kits, respectively. The binding of miR-361-3p and MTCP1 were detected by luciferase reporter assay. Results Relative to NHA, miR-361-3p levels were lower in LN299 and U87MG. Transfected with mimic, MTCP1 levels and the proliferation of U87MG cells were decreased, and the Caspase3 activity was increased, and luciferase activity in WT-MTCP1 was decreased. Furthermore, transfected with inhibitor, the proliferation of U87MG cells were increased, while the apoptosis rate in the si-MTCP1 group was increased. Conclusion MiR-361-3p may inhibit the proliferation and promote apoptosis of GB cells by inhibiting MTCP1.

Key words： Glioblastoma Cell proliferation Apoptosis miR-361-3p

基金资助:国家自然科学基金青年项目（81602207、81802502）；
陕西省自然科学基础研究计划项目（2018JM7062）。

通讯作者: 宋锦宁（1962.9-），男，博士，教授，主任医师，博士生导师；研究方向：脑血管病、脑肿瘤基础与临床。

作者简介: 郭小叶（1976.10-），女，西安交通大学医学院2016级临床医学专业在读博士研究生；研究方向：脑胶质瘤基础与临床。

引用本文:

郭小叶冒平王佳廉海平王伟白晓斌宋锦宁. miR-361-3p调节恶性胶质瘤细胞功能的研究[J]. 中国医药导报, 2019, 16(28): 7-11.
GUO Xiaoye MAO Ping WANG Jia LIAN Haiping WANG Wei BAI Xiaobin SONG Jinning. Studies of miR-361-3p in regulating the function of glioblastoma cells. 中国医药导报, 2019, 16(28): 7-11.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2019/V16/I28/7

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