microRNA-182 在口腔鳞癌细胞中的表达及对细胞生物学行为的影响

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摘要目的检测microRNA-182（miR-182）在口腔鳞癌组织中的表达，探讨miR-182对口腔鳞癌细胞增殖和迁移能力的影响。方法收集2015年7月~2016年10月哈尔滨医科大学附属口腔医院住院患者10例鳞状细胞癌标本和配对组织，实时荧光定量PCR方法检测miR-182 mRNA在口腔鳞癌组织中的表达水平。培养舌鳞状细胞癌细胞系TCA8113，分四组：空白对照组（Control组）、空载体组（Vehicle组）、阴性对照组（miR-182-NC组）和miR-182-inhibitor组（miR-182-i组）。利用MTT法和Transwell小室方法分别检测TCA8113细胞转染miR-182-inhibitor后的增殖和迁移能力的变化。结果口腔鳞癌组织与配对组织比较，miRNA-182的表达显著增高（P < 0.05）。转染miR-182-inhibitor后，miR-182-i组细胞的增殖能力和细胞迁移数显著低于Control组、（P < 0.05）。结论 miR-182的高表达可能与口腔鳞癌的发生发展有关，下调miR-182的表达可抑制TCA8113细胞的增殖迁移能力，其作用机制尚需进一步研究。

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	王飞飞安晶红宋美王金辉▲

关键词 ：口腔鳞状细胞癌, 微小RNA, miRNA-182

Abstract：Objective To investigate the expression of miRNA-182 (miR-182) in oral squamous cell carcinoma (OSCC) and explore the effects of miR-182 deficiency on proliferation and migration in OSCC cells. Methods 10 cases of OSCC samples and paired tissues were collected from Stomatological Hospital of Harbin Medical University from July 2015 to October 2016. The expression of miR-182 in OSCC tissues was detected by quantitative reverse-transcription polymerace chain reaction (RT-qPCR). Culturing TCA8113 cells and separating the samples to four groups: Control, Vehicle, miR-182-NC and miR-182-i group. After transfection with miR-182 inhibitor, TCA8113 cell proliferation and migration were examined with MTT assay and Transwell assay. Results The level of miR-182 in OSCC samples was significantly higher than that in normal paired tissue group (P < 0.05). The proliferation capability and migration capability in miR-182-i group were decreased than those in Control group (P < 0.05). Conclusion The high expression of miR-182 may be correlated with the development of OSCC. Decreased the expression of miR-182 may inhibite OSCC cancer cell proliferation and migration. Further study should be conducted to explore the function of miR-182 in OSCC development.

Key words： Oral squamous cell carcinoma miRNA miRNA-182

基金资助:黑龙江省哈尔滨市第一医院人才引进项目（2014 SYYRCYJ03）。

通讯作者: ▲通讯作者

作者简介: 王飞飞（1989-），女，硕士研究生；研究方向：肿瘤的靶向检测

引用本文:

王飞飞安晶红宋美王金辉▲. microRNA-182 在口腔鳞癌细胞中的表达及对细胞生物学行为的影响[J]. 中国医药导报, 2017, 14(34): 17-20.
WANG FeiFei AN Jinghong SONG Mei WANG Jinhui▲. Expression of miR-182 in oral squamous cell carcinoma and its effect on cell biological behaviour. 中国医药导报, 2017, 14(34): 17-20.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2017/V14/I34/17

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