Significances of changes of matrix metalloproteinases-13, TNF-α in serums and synovial fluids of patients with knee osteoarthritis
WANG Lei1 DONG Hong2 KONG Jichang3 Yang Jihong4
1.Department of Orthopedics and Traumatology, Traditional Chinese Medicine Hospital of Chengde, Hebei Province, Chengde 067000, China;
2.First Department of Orthopaedics, North China Petroleum General Hospital, Hebei Province, Renqiu 062552, China;
3. Department of Medicine, North China Petroleum General Hospital, Hebei Province, Renqiu 062552, China;
4. Department of Urology, North China Petroleum General Hospital, Hebei Province, Renqiu 062552, China
Abstract：Objective To explore the changes of matrix metalloproteinases 13 (MMP-13), TNF-α in serums and synovial fluids of patients with knee osteoarthritis (KOA). Methods From May 2014 to May 2016, in Traditional Chinese Medicine Hospitals of Chengde, 84 patients with KOA and 80 healthy persons (control group) were selected, according to Kellgren & Lawrence (K-L) grading, they were divided into KOA 1~2 (n = 45) and KOA 3~4 (n = 39). the levels of TNF-α, MMP-13 in the serums and synovial fluids were measures by ELISA. Results The WOMAC score of KOA 3～4 patients was higher than KOA1～2 patients, the difference was statistically significant (P < 0.05); the levels of TNF-α, MMP-13 in serum and synovial fluid of KOA 3～4 patients were higher than KOA 1～2 patients, the differences were statistically significant (P < 0.05). the level of MMP-13 in serum and synovial fluid of KOA 1～2 patients were higher than control group, the differences were statistically significant (P < 0.05), but there was no statistically significant difference in the TNF-α levels (P > 0.05). The level of MMP-13 in serum of KOA patients was higher than synovial fluid, the difference was statistically significant (P < 0.05), but there was no statistically significant difference in TNF-α levels (P > 0.05). Correlation analysis showed that the serum and synovial fluid levels of MMP-13 in KOA patients were positively correlated with WOMAC score (r = 0.599, 0.641, P < 0.05), but the serum and synovial fluid levels of TNF-α in KOA patients were no correlated with WOMAC score (P > 0.05). Conclusion The serum and synovial fluids levels of TNF-α and MMP-13 elevate in KOA patients, and then MMP-13 levels show correlation with WOMAC scores.
王磊1 董红2 孔继昌3 杨继宏4. 膝骨关节炎患者血清和关节液中MMP-13和TNF-α水平的变化意义[J]. 中国医药导报, 2017, 14(15): 98-101.
WANG Lei1 DONG Hong2 KONG Jichang3 Yang Jihong4. Significances of changes of matrix metalloproteinases-13, TNF-α in serums and synovial fluids of patients with knee osteoarthritis. 中国医药导报, 2017, 14(15): 98-101.
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2017, Vol. 14 
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