Abstract：Objective To establish the quality control method of Xinhui Citri Reticulatae Pericarpium. Methods The chromatographic conditions of high performance liquid chromatography (HPLC) were Atlantis T3-C18 column (4.6 mm × 250.0 mm, 5 μm), and mobile phase was acetonitrile-0.5% acetic acid water system, gradient elution was at a flow rate of 1.0 mL/min. The detection wavelength was set at 283 nm and 330 nm. The contents of hesperidin, nobiletin and tangeretin in 46 batches of Xinhui Citri Reticulatae Pericarpium with different origins, different harvesting time and different aging time were detected. The ratio range was calculated. Pearson correlation analysis was performed on the aged years and the contents of the three compounds. Results The hesperidin content was not less than 2.56%, the nobiletin content was not less than 0.27%, and the tangeretin content was not less than 0.18%, and the ratio range of the three flavonoids was 100∶(7.58-22.09)∶(4.89-14.55). There was no correlation between the aging of aging and hesperidin or nobiletin. The age of aging was negatively correlated with hesperidin (r = -0.345, P ＜ 0.05); there was a positive correlation among hesperidin, nobiletin and tangeretin (r = 0.544, 0.581, 0.873, all P ＜ 0.01). Conclusion The limited standards and ratio ranges of these three compounds could control the quality of Xinhui Citri Reticulatae Pericarpium effectively.
2019, Vol. 16 
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