Effect of high risk HPV infection on the genes expression of apoptosis and proliferation in cervical intraepithelial neoplasia
TAO Minfeng1 WANG Xiaorong2
1.Health Department, Beijing Maternal and Child Health Care Hospital, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China; 2.Department of Perinatal Medicine, Beijing Maternal and Child Health Care Hospital, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China
Abstract：Objective To study the effect of high risk HPV infection on the expression of apoptosis and proliferation genes in cervical intraepithelial neoplasia (CIN). Methods CIN tissues and inflammatory lesions tissues received biopsy in Beijing Maternal and Child Health Care Hospital, Beijing Obstetrics and Gynecology Hospital, Capital Medical University from May 2013 to December 2016 were collected, respectively enrolled in CIN group and control group. CIN tissues were divided into <1 RLU/CO group, 1-100 RLU/CO group, >100-1000 RLU/CO group, >1000 RLU/CO group according to HPV-DNA RLU/CO. Meanwhile, cell cycle protein and its kinase, inhibitor of apoptosis proteins in cervical tissue were determined. Results Expression levels of cyclinB1, cyclinD1, cyclinE, CDK4, CDK6, Apollon, c-IAP1, Survivin, Livin mRNA in cervical tissue of CIN group were significantly higher than those of control group, with statistically significant differences (P < 0.05). Expression levels of cyclinB1, cyclinD1, cyclinE, CDK4, CDK6, Apollon, c-IAP1, Survivin, Livin mRNA in CIN cervical tissue of 1-100 RLU/CO group, >100-1000 RLU/CO group, >1000 RLU/CO group were significantly higher than those of the <1 RLU/CO group, and the higher the HPV-DNA RLU/CO, the higher the expression of cyclinB1, cyclinD1, cyclinE, CDK4, CDK6, Apollon, c-IAP1, Survivin, Livin mRNA, with statistically significant differences (P < 0.05). Conclusion High risk HPV infection in cervical intraepithelial neoplasia can increase the expression of cyclin and its kinase and inhibitor of apoptosis protein genes.
陶旻枫1 王小榕2. 宫颈上皮内瘤变组织中高危型HPV感染对凋亡、增殖基因表达的影响[J]. 中国医药导报, 2017, 14(17): 80-83,91.
TAO Minfeng1 WANG Xiaorong2. Effect of high risk HPV infection on the genes expression of apoptosis and proliferation in cervical intraepithelial neoplasia. 中国医药导报, 2017, 14(17): 80-83,91.
[1] Khan MJ，Castle PE，Lorincz AT，et al. The elevated 10-year risk of cervical precancer and cancer in women with human papillomavirus（HPV）type 16 or 18 and the possible utility of type-specific HPV testing in clinical practice [J]. J Natl Cancer Inst，2015，97（14）：1072-1079.
[2] Castellsagué X，Pawlita M，Roura E，et al.Prospectiveseroepi-demiologic study on the role of Human Papillomavirus and other infections in cervical carcinogenesis：evidence from the EPIC cohort [J]. Int J Cancer，2014，135（2）：440-452.
[3] Zhang R，Shen C，Zhao L，et al. Dysregulation of host cellular genes targeted by human papillomavirus（HPV）integration contributes to HPV-related cervical carcinoge-nesis [J]. Int J Cancer，2016，138（5）：1163-1174.
[4] 蒋燕明，李力.宫颈上皮内瘤变进展相关基因的生物信息学分析[J].中国肿瘤临床，2016，43（19）：840-843.
[5] 陈锐，赵健，朱丽荣，等.人类乳头状瘤病毒分型检测在宫颈癌筛查中的临床研究[J].中华实验和临床病毒学杂志，2014，28（6）：429-432.
[6] Zhao LW，Zhong XH，Yang SY，et al. Inotodiol inhabits proliferation and induces apoptosis through modulating expression of cyclinE，p27，bcl-2，and bax in human cervical cancer HeLa cells [J]. Asian Pac J Cancer Prev，2014，15（7）：3195-3199.
[7] Omori M，Hashi A，Kondo T，et al. Dysregulation of CDK inhibitors and p53 in HPV-negative endocervical adeno-carcinoma [J]. Int J Gynecol Pathol，2015，34（2）：196-203.
[8] Chalertpet K，Pakdeechaidan W，Patel V，et al. Human papi-llomavirus type 16 E7 oncoprotein mediates CCNA1 promoter methylation [J]. Cancer Sci，2015，106（10）：1333-1340.
[9] Griffin H，Soneji Y，Van Baars R，et al. Stratification of HPV-induced cervical pathology using the virally encoded molecular marker E4 in combination with p16 or MCM [J]. Mod Pathol，2015，28（7）：977-993.
[10] 郑健，王琨.CyclinB1、HPV16 蛋白在宫颈组织芯片中的表达及临床意义[J].中国老年学杂志，2016，36（5）：1050-1052.
[11] Umbreit C，Erben P，Faber A，et al. MMP9，Cyclin D1 and β-Catenin are useful markers of p16-positive squamous cell carcinoma in therapeutic EGFR inhibition in vitro [J]. Anticancer Res，2015，35（7）：3801-3810.
[12] Stewart CJ，Crook ML. Cervical intraepithelial neoplasia（CIN）3-like squamous cell carcinoma of the cervix：a review of 14 cases with comparison of E-cadherin and cyclin D1 expression in the CIN 3-like and infiltrative tumour elements [J]. Histopathology，2017，70（3）：367-374.
[13] 林苏瑜，徐曙光，章洁琳.凋亡抑制蛋白Apollon在宫颈癌组织中的表达及意义[J].中国妇幼保健，2015，30（19）：3291-3292.
[14] Milani S，Bandehpour M，Sharifi Z，et al. Suppressive Effect of Constructed shRNAs against Apollon Induces Apoptosis and Growth Inhibition in the HeLa Cell Line [J]. Iran Biomed J，2016，20（3）：145-151.
[15] 朱明玥，陈帆，高冬梅，等.c-IAP1在宫颈癌中的表达及临床意义[J].中国妇幼保健，2014，29（14）：2253-2257.
[16] Liu HQ，Wang YH，Wang LL，et al. P16INK4A and survivin：Diagnostic and prognostic markers in cervical intraepit-helial neoplasia and cervical squamous cell carcinoma [J]. Exp Mol Pathol，2015，99（1）：44-49.
[17] Xu M，Xia LP，Fan LJ，et al. Livin and caspase-3 expression are negatively correlated in cervical squamous cell cancer [J]. Eur J Gynaecol Oncol，2013，34（2）：152-155.
[18] Johnson DC，Bhatta MP，Smith JS，et al. Assessment of high-risk human papillomavirus infections using clinician-and self-collected cervical sampling methods in rural women from far western nepal [J]. PLoS One，2014，9（6）：e101255.
[19] 林苏瑜.LEEP手术中不同宫颈切除深度对宫颈上皮内瘤变治疗效果的影响[J].中国医药科学，2016，6（12）：98-100，152.
[20] 蒋桔莲，柴泽英.重组干扰素α-2b栓在高危型 人乳头瘤病毒感染宫颈上皮内瘤变患者行宫颈环形电切术后的应用[J].中国性科学，2016，25（2）：76-78.DOI：10.3969/j.issn.1672-1993.2016.02.026.
[21] 李玉霞，张燕.高危型HPV筛查联合液基细胞学检测在宫颈癌筛检中的应用价值[J].海南医学院学报，2014， 20（2）：223-225.
[22] 李晖，王仙荣.HPV检测在宫颈上皮内瘤变术后复发预测上的意义探究[J].中国性科学，2016，25（1）：53-56.DOI：10.3969/j.issn.1672-1993.2016.01.016.
[23] 钟继兴.高危型HPV-DNA检测在宫颈癌筛查中的应用[J].中国医药科学，2015，5（15）：144-146.
[24] 白彩云，王延明.宫颈上皮内瘤变患者高危型HPV感染与潜在恶性生物学行为的关系[J].海南医学院学报，2015，21（9）：1290-1293.
2017, Vol. 14 
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