Study on protective effect of bakuchiol on photoaging HSF cells
LIU Guoliang1 YAO Yuan1 ZHANG Ning1 GUO Wei2 WANG Yao2 LIU Bin1
1.College of Jiamusi, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Jiamusi 154007, China;
2.the First Clinical Medical College, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China
Abstract：Objective To study the protective effect of bakuchiol on photoaging human skin fibroblasts (HSF) induced by ultraviolet radiation B (UVB) irradiation. Methods HSF cells were cultured in vitro and divided into blank group (normal culture, no UVB radiation), UVB model group (UVB radiation), estradiol group and bakuchiol low (1 μmol/L), medium (10 μmol/L) and high dose (100 μmol/L) groups. Cell viability was detected by MTT assay. The protein expressions of type Ⅰ collagen (COL Ⅰ), matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-3 (MMP-3) were detected by Western blot. The expression levels of COL Ⅰ and MMP-1 mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR). Results The optimal UVB radiation dose for photoaging HSF cell model was 25 mJ/cm2. Compared with the blank group, there was no significant difference in cell viability and secretion of MMP-1, MMP-3 and COL Ⅰ between the low, medium and high dose groups of bakuchiol (P > 0.05); the cell viability and COL Ⅰ expression level in the UVB model group decreased significantly (P < 0.05), the protein expression levels of MMP-1 and MMP-3 increased significantly (P < 0.05); the mRNA expression levels of MMP-1 and COL Ⅰ decreased significantly (P < 0.05). Compared with the UVB model group, the cell viability and COL Ⅰ expression in the low, middle and high dose groups of bakuchiol increased significantly (P < 0.05), the protein expression levels of MMP-1 and MMP-3 decreased significantly (P < 0.05), the mRNA expression levels of MMP-1 and COL Ⅰ increased significantly (P < 0.05). Conclusion Bakuchiol can promote the expression of COL Ⅰ in HSFs, inhibit the expression of MMP-1 and MMP-3, protect UVB from damage to HSFs and exert anti-photoaging effects on skin.
刘国良1 姚远1 张宁1 郭玮2 王瑶2 刘斌1. 补骨脂酚对光老化HSF细胞保护作用研究[J]. 中国医药导报, 2019, 16(19): 11-15,24.
LIU Guoliang1 YAO Yuan1 ZHANG Ning1 GUO Wei2 WANG Yao2 LIU Bin1. Study on protective effect of bakuchiol on photoaging HSF cells. 中国医药导报, 2019, 16(19): 11-15,24.
2019, Vol. 16 
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