Effect of trichosanthin on proliferation and apoptosis of human cervical cancer cells and research on its mechanism
WANG Fan1,2 DENG Xinyan1,2 YAO Minghui1 TIAN Tian1 LIU Ying1 WANG Hui1 WANG Jia1 DONG Xiaoyun1,2▲
1.Medical College of Yangzhou University, Jiangsu Province, Yangzhou 225000, China;
2.Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Jiangsu Province, Yangzhou 225000, China
Abstract：Objective To study the effect and mechanism of trichosanthin (TCS) on inducing apoptosis of human cervical cancer HeLa cells. Methods HeLa cells were treated with low (7 μmol/L), medium (14 μmol/L) and high (28 μmol/L) doses of TCS for 10-82 h, the cell morphology and inhibition of cell growth was observed. The cell cycle and apoptosis was detected and Western blot was performed to detect the protein expression of apoptosis proteins of cervical cancer cells HeLa after treated with TCS for 24 h. Results HeLa cells of cervical cancer treated by TSC showed wrinkles and deformation, the growth of HeLa cells was inhibited. The number of cells in low and medium dose of TCS group decreased with time (r = -0.9727, -0.9757, P ＜ 0.05). Compared with the blank control group, the G0/G1 phase of HeLa cells in medium, high dose of TCS group were significantly increased (P < 0.05), and the S phase in low, medium, high dose of TCS group were decreased after TCS treated for 24 h, the differences were statistically significant (P ＜ 0.05). The apoptosis rate of HeLa cells in the low, medium and high dose of TCS group was increased compared with that in the blank control group (P ＜ 0.05). Compared with the blank control group, the protein expressions levels of Caspase-9, Caspase-3, polyadenosine diphosphate ribosome polymerase (PARP) and p-extracellular regulated protein kinase (ERK) in low, medium, high dose of TCS group were gradually decreased in a dose-dependent manner after of TCS treatment (r = -0.969, P ＜ 0.05). Conclusion TCS can induce apoptosis by activating the Caspase family of proteins to inhibit the proliferation of cervical cancer HeLa cells to exert anti-tumor effects.
王凡1,2 邓心燕1,2 姚铭辉1 田甜1 刘影1 王慧1 王佳1 董小耘1,2▲. 天花粉蛋白对人宫颈癌细胞增殖及凋亡的影响及其机制研究[J]. 中国医药导报, 2019, 16(17): 13-17.
WANG Fan1,2 DENG Xinyan1,2 YAO Minghui1 TIAN Tian1 LIU Ying1 WANG Hui1 WANG Jia1 DONG Xiaoyun1,2▲. Effect of trichosanthin on proliferation and apoptosis of human cervical cancer cells and research on its mechanism. 中国医药导报, 2019, 16(17): 13-17.
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2019, Vol. 16 
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