Effect of white grub extract on the expression of MMP-9 of experimental rabbits with retinal vein occlusion in different time windows
JIANG Pengfei1,2 MA Junxu1,2 PENG Jun2,3 PENG Qinghua1,2,3
1.College of Traditional Chinese Medicine, Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410208, China;
2.Hu′nan Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases, Hu′nan Province, Changsha 410208, China;
3.Department of Ophthalmology, the First Affiliated Hospital of Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410007, China
Abstract：Objective To evaluate the therapeutic effect and mechanism of white grub extract on experimental rabbits with retinal vein occlusion (RVO) in different time windows. Methods Forty rabbits were randomly divided into 4 groups: group A, group B, group C, group D, with 10 rabbits and 20 eyes in each group. Except for group A, photochemical kinetic method was used to establish RVO animal model in the other three groups. Group A and B were intragastrically administered with 5 mL/kg of normal saline. Group C was intragastrically administered with 5 mL/kg of Compound Xueshuantong Suspension, and group D was intragastrically administered with 1.5 mL/kg of white grub extract. After administration for 1 and 3 weeks, fluorescein fundus angiography (FFA) was examined to calculate to the ratio of area of non-perfusion area and area of optic disk, HE staining was used to observe the retinal morphology, immunohistochemistry was used to detect the expression of matrix metalloproteinase (MMP)-9. Results The results of FFA test showed that the ratio of area of non-perfusion area and area of optic disk in group C and D was significantly lower than that of group B after modeling for 1 week and 3 weeks, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The results of light microscopy of retinal sections showed that in group B, 1 week after modeling, the retinal tissue was edematous, the cells were arranged in disorder, and there was no new vascular cord, after 3 weeks of modeling, a large number of neovascular cords appeared; in group C, 1 week after modeling, the retinal tissue was edematous, the cells were arranged in disorder, 3 weeks after modeling, the edema of retinal tissue was reduced, there was no neovascular cord; in group D, 1 week after modeling, the retinal tissue cells were roughly arranged, 3 weeks after modeling, the edema of retinal tissue was reduced, there was no neovascular cord. The results of MMP-9 immunohistochemistry showed that the expression of MMP-9 was up-regulated in each group, group D was the weakest, followed by group C, and group B was the strongest, the expression of MMP-9 in group B, C and D was higher than that of group A, while the expression of MMP-9 in group C and D was lower than that of group B, the differences were all statistically significant (P ＜ 0.05). Conclusion White grub extract can improve the state of retinal ischemia and hypoxia and inhibit the expression of MMP-9 in the experimental RVO model.
蒋鹏飞1,2 马俊旭1,2 彭俊2,3 彭清华1,2,3. 蛴螬提取物对实验性兔视网膜静脉阻塞不同时间窗MMP-9表达的影响[J]. 中国医药导报, 2019, 16(15): 8-11.
JIANG Pengfei1,2 MA Junxu1,2 PENG Jun2,3 PENG Qinghua1,2,3. Effect of white grub extract on the expression of MMP-9 of experimental rabbits with retinal vein occlusion in different time windows. 中国医药导报, 2019, 16(15): 8-11.
[1] 邱晓星，彭清华，陈梅，等.蛴螬提取物对兔脉络膜新生血管中Ang1和PEDF表达的影响[J].国际眼科杂志，2012，12（11）：2053-2058.
[2] 张波涛，彭清华，叶群如，等.蛴螬提取物对兔视网膜静脉阻塞模型视网膜组织ET-1表达的影响[J].湖南中医药大学学报，2012，32（9）：8-11.
[3] 阳长明，侯世祥，罗英杰，等.蛴螬滴眼液中氨基酸成分的测定[J].湖南中医杂志，2001，32（1）：57-59.
[4] 陈立军，王雨生.“二步法”激光诱导脉络膜视网膜静脉吻合术治疗兔视网膜中央静脉阻塞的视觉电生理变化[J].眼科新进展，2006，26（4）：253.
[5] 唐坤，李素芬.视网膜分支静脉阻塞无灌注区范围、视野阈值与新生血管关系的研究[J].国际眼科杂志，2009，9（6）：15-16.
[6] Wolf-Schnurrbusch U E K. Retinal Vein Occlusion[M]//Spectral Domain Optical Coherence Tomography in Macular Diseases. New Delhi：Springer，2017：147-150.
[7] Goldenberg D，Loewenstein A. Retinal Vein Occlusion[M]//Medical Retina. Karger Publishers，2017：32-41.
[8] Jonas JB，Monés J，Glacet-Bernard A，et al. Retinal vein occlusions [M]// Macular Edema. Karger Publishers，2017：139-167.
[9] Starr MR，Norby SM，Scott JP，et al. Acute retinal vein occlusion and cystic fibrosis [J]. Int J of Ret，2018，4（1）：26.
[10] Prajapati VA，Vasavada D，Patel SM，et al. A study of evaluation of various risk factors of retinal vein occlusion [J]. Int J Res Med Sci，2017，2（3）：1054-1057.
[11] Bucciarelli P，Passamonti SM，Gianniello F，et al. Thrombophilic and cardiovascular risk factors for retinal vein occlusion [J]. Eur J Intern Med，2017，44：44-48.
[12] Winegarner A，Wakabayashi T，Hara-Ueno C，et al. Retinal microvasculature and visual acuity after intravitreal aflibercept in eyes with central retinal vein occlusion：an optical coherence tomography angiography study [J]. Retina，2018，38（10）：2067-2072.
[13] 倪素娜，刘静.基质金属蛋白酶及组织抑制物与Toll样受体在胎膜早破患者胎膜组织中的表达[J].河北医药，2018，40（1）：28-31.
[14] Carpio LR，Bradley EW，Westendorf JJ. Histone deacetylase 3 suppresses Erk phosphorylation and matrix metalloproteinase（Mmp）-13 activity in chondrocytes [J]. Connect Tissue Res，2017，58（1）：27-36.
[15] Manicone AM，Gharib SA，Gong KQ，et al. Matrix Metalloproteinase-28 is a key contributor to emphysema pathogenesis [J]. Am J Path，2017，187（6）：1288-1300.
[16] Lebre MC，Vieira PL，Tang MW，et al. Synovial IL-21/TNF- producing CD4+ T cells induce joint destruction in rheumatoid arthritis by inducing matrix metalloproteinase production by fibroblast-like synoviocytes [J]. J Leukocyte Biol，2017，101（3）：775-783.
[17] Naveed S，Clements D，Jackson DJ，et al. Matrix metalloproteinase-1 activation contributes to airway smooth muscle growth and asthma severity [J]. Am J Resp and Crit Care，2017，195（8）：1000-1009.
[18] Chao HM，Chao WJ，Chen L. Retinal Ischemia：MMP-9；Its Relation to Resveratrol，Baicalein，S-allyl L-cysteine and Chi Ju Di Huang Wan [M]// The Role of Matrix Metalloproteinase in Human Body Pathologies. InTech，2017.
[19] Zhu SH，Liu BQ，Hao MJ，et al. Paeoniflorin suppressed high glucose-induced retinal microglia MMP-9 expression and inflammatory response via inhibition of TLR4/NF-κB pathway through upregulation of SOCS3 in diabetic retinopathy [J]. Inflammation，2017，40（5）：1475-1486.
[20] Halder K，Banerjee S，Ghosh S，et al. Mycobacterium indicus pranii（Mw）inhibits invasion by reducing matrix metalloproteinase（MMP-9）via AKT/ERK-1/2 and PKCα signaling：A potential candidate in melanoma cancer therapy [J]. Cancer Biol Ther，2017，18（11）：850-862.
[21] Li W，Suwanwela NC，Patumraj S. Curcumin prevents reperfusion injury following ischemic stroke in rats via inhibition of NF-κB，ICAM-1，MMP-9 and caspase-3 expression [J]. Mol Med Rep，2017，16（4）：4710-4720.
2019, Vol. 16 
京公网安备 11010502046598号