Effect and mechanism of Allicin inhibition on human papillary thyroid carcinoma TPC-1 cells
ZHAI Jian1 HAN Xiaochen1▲ YAN Jinyin2
1.Department of Head and Neck Tumor Surgery, Tangshan People′s Hospital, Hebei Province, Tangshan 063001, China;
2.Department of Breast surgery, Tangshan People′s Hospital, Hebei Province, Tangshan 063001, China
Abstract：Objective To investigate the effect and mechanism of Allicin inhibition on the growth of human thyroid papillary carcinoma TPC-1 cells. Methods The cultured TPC-1 cells were divided into the control group and the Allicin treatment groups with different concentrations (10, 20, 30, 40, 50 μg/mL). Methylthiazoletrazolium (MTT) method was used to determine the inhibitory effect of different concentrations of Allicin treatment on the growth of TPC-1 cells at 24, 48, 72 h. Inverted microscope and scanning electron microscope were used to observe the change of cell morphology and ultrastructure after treatment by Allicin. Flow cytometry was used to test the effects of Allicin on cell cycle. Immunocytochemical technique was used to detect the effects on expression of intracellular apoptotic protein Bax and anti-apoptotic protein Bcl-2 after treatment by Allicin. Results Compared with the control group, different concentrations of Allicin could inhibit the growth of TPC-1 cells, the differences were statistically significant (P ＜ 0.05); the inhibition rate of Allicin on cells was related to time and concentration. With the increase of Allicin concentration, the proportion of G2/M phase cells was significantly increased, while that of G0/G1 phase cells was decreased. All pairwise comparison of adjacent concentration groups showed statistically significant differences (P ＜ 0.05) except the control group and 10 μg/mL Allicin group (P ＞ 0.05). Compared with the control group, the intracellular Bcl-2 expression was decreased and Bax expression was increased in every concentration of Allicin treatment group, the differences were statistically significant (P ＜ 0.05); the expression levels of Bcl-2 and Bax in Allicin treatment groups with different adjacent concentrations were compared, the differences were statistically significant (P ＜ 0.05). Conclusion Allicin can inhibit the growth of TPC-1 cells of papillary thyroid cancer, and its mechanism may be related to inhibition of cell proliferation by Allicin and the induction of apoptosis by down-regulating Bcl-2 and up-regulating Bax.
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