Effects of Bawei Longzuan Granules in rats with adjuvant arthritis
LUO Yudong1,2 WU Yuqiang1 PANG Yuzhou2,3 TAN Anqiang1 MI A'na3
1.Pharmaceutical Factory, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530023, China;
2.Key Laboratory of Zhuang Medicine Prescriptions Basis and Application Research of Guangxi Colleges and Universities, Guangxi Zhuang Autonomous Region, Nanning 530001, China;
3.College of Zhuang Medicine, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530001, China
Abstract:Objective To study the effects of Bawei Longzuan Granules in rats with adjuvant arthritis. Methods Sixty SD rats were randomly divided into blank control group, model group, Tripterygium Wilfordii Tablets group, and low, medium, high dose of Bawei Longzuan Granules group. Except for blank control group, the other groups were only given 0.1 mL of Freund complete adjuvant (FCA) injection into the left-hind feet of each rat. At the eighth day of the experiment, intragastric administration was started, once a day, the dosing volume was 10 mL/kg, the dosages of Bawei Longzuan Granules were 5.9, 11.8, 23.6 g/kg, the dosage of Tripterygium Wilfordii Tablets was 0.009 45 g/kg, blank control group and model group were given same volume of distilled water, continuous intragastric administration for 21 days. The conditions of ankle joint swelling and pathologic changes in model rats with adjuvant arthritis were observed, and enzyme-linked immunosorbent assay (ELISA) was used to detect the contents of interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α) in serum and joint tissue homogenate of rats. Results Compared with blank control group, started from the eighth day of making model, the feet thickness of model group and all medication groups was increased, the differences were statistically significant (P < 0.01 or P < 0.05); compared with model group, the swelling degree of toes in high dose of Bawei Longzuan Granules group at the 29th day of modeling was decreased, the difference was statistically significant (P < 0.05); the pathological examination showed that the proliferative degree of synovial cells and the invasive degree of inflammatory cells in high dose of Bawei Longzuan Granules group were all reduced; there were no statistically significant differences of the contents of serum IL-1β, TNF-α in each dose of Bawei Longzuan Granules group compared with those of model group (P > 0.05). Conclusion Bawei Longzuan Granules can decrease the swelling degree of toes in rats with adjuvant arthritis, reduce the proliferation of synovial cells and inflammatory cell infiltration, which has certain improving effects for the pathological model of rats with adjuvant arthritis.
罗宇东1,2 吴玉强1 庞宇舟2,3 谭安蔷1 米阿娜3. 八味龙钻颗粒对佐剂性关节炎大鼠的影响[J]. 中国医药导报, 2017, 14(33): 26-30.
LUO Yudong1,2 WU Yuqiang1 PANG Yuzhou2,3 TAN Anqiang1 MI A'na3. Effects of Bawei Longzuan Granules in rats with adjuvant arthritis. 中国医药导报, 2017, 14(33): 26-30.