A preliminary study on the cross blood-brain-barrier transport of exosomal microRNA-135a
LIU Chengeng1 HAO Ting2 YANG Tingting1 MENG Shuang3 WANG Peichang1
1.Department of Clinical Laboratory, Xuanwu Hospital, Capital Medcial University, Beijing 100053, China;
2.Department of Pathology, Heze Municipal Hospital, Shandong Province, Heze 274000, China;
3.State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract：Objective To study the effect of microRNA-135a (miR-135a) on blood-brain barrier and cell transport in exocrine. Methods The high miR-135a exosomes were extracted from the cerebrospinal fluid (CSF) of the transgenic mice and injected into the ventricles of the wild type mice and intervened with SH-SY5Y cells. The levels of miR-135a in the peripheral blood of mice and the exosomes of the culture, and the expression and activity of β-secretase-1 (BACE-1) in SH-SY5Y cells were determined. Results Intraventricular injection of miR-135a exosomes resulted in a significant increase in CSF and plasma exosomal miR-135a in wild-type mice (P < 0.05). The activity of BACE-1 in SH-SY5Y cells treated with exosomes harvest from the CSF of APP/PS1 double transgenic mice was significantly lower than that in other intervention group and control group (P < 0.05), and the mRNA expression level of SH-SY5Y cells was significantly lower than that of control (P < 0.05). The trend of change is consistent with the trend of activity. Conclusion The signal of "high miR-135a" can be transferred from CSF to blood through the blood-brain barrier, which provides a more solid experimental basis for the use of exosomal miR-135a as a biomarker of Alzheimer's disease.
刘辰庚1 郝婷2 杨婷婷1 孟双3 王培昌1. 外泌体内microRNA-135a跨血脑屏障转运的[J]. 中国医药导报, 2017, 14(33): 22-25,30.
LIU Chengeng1 HAO Ting2 YANG Tingting1 MENG Shuang3 WANG Peichang1. A preliminary study on the cross blood-brain-barrier transport of exosomal microRNA-135a. 中国医药导报, 2017, 14(33): 22-25,30.
[1] Liu CG，Wang JL，Li L，et al. MicroRNA-135a and-200b，potential biomarkers for Alzheimer′s disease，regulate β secretase and amyloid precursor protein [J]. Brain Res，2014， 1583：55-64.
[2] 王全全，吕占云，孙大芳，等.早发家族性阿尔茨海默病家系患者血清miRNA检测及初步分析[J].中华行为医学与脑科学杂志，2017，26（6）：554-559.
[3] 刘辰庚，霍贵申，孟双，等.MicroRNA-135a突变体对β分泌酶-1的调控作用及初步临床调查[J].临床和实验医学杂志，2016，15（24）：2389-2392.
[4] 刘辰庚，孟双，张跃其，等.MicroRNA-135a在阿尔茨海默病患者中的检测[J].中国老年学杂志，2016，36（1）：152-153.
[5] Quinlan S，Kenny A，Medina M，et al. MicroRNAs in neurodegenerative diseases [J]. Int Rev Cell Mol Biol，2017， 334：309-43.
[6] Erdal ME，Y?覦lmaz SG，Gürgül S，et al. MiRNA expression profile is altered differentially in the rat brain compared to blood after experimental exposure to 50 Hz and 1 mT electromagnetic field [J]. Prog Biophys Mol Biol，2017. pii： S0079-6107（17）30147-5. doi：10.1016/j.pbiomolbio.2017. 08.001.[Epub ahead of print]
[7] Riancho J，Vázquez-Higuera JL，Pozueta A，et al. MicroRNA profile in patients with Alzheimer's disease：analysis of miR-9-5p and miR-598 in raw and exosome enriched cerebrospinal fluid samples [J]. J Alzheimers Dis，2017，57（2）：483-491.
[8] 贾建平，陆璐，张逸驰，等.美国国立老化研究所与阿尔茨海默病协会诊断指南写作组：阿尔茨海默病源性轻度认知障碍诊断标准推荐[J].中华神经内科杂志，2012，45（5）：345-351.
[9] 贾建平，陆璐，张逸驰，等.美国国立老化研究所与阿尔茨海默病协会诊断指南写作组：阿尔茨海默病痴呆诊断标准的推荐[J].中华神经内科杂志，2012，45（5）：352-355.
[10] Putteeraj M，Yahaya MF，Teoh SL. MicroRNA dysregulation in Alzheimer′s disease [J]. CNS Neurol Disord Drug Targets，2017.doi：10.2174/1871527316666170807142311.
[11] Silva GJJ，Bye A，El Azzouzi H，et al. MicroRNAs as important regulators of exercise adaptation [J]. Prog Cardiovasc Dis，2017，60（1）：130-151.
[12] Recabarren D，Alarcón M. Gene networks in neurodegenerative disorders [J]. Life Sci，2017，183：83-97.
[13] Hu YB，Li CB，Song N，et al. Diagnostic value of microRNA for Alzheimer's Disease：a systematic review and meta-analysis [J]. Front Aging Neurosci，2016，8：13.
[14] Wang X，Liu D，Huang HZ，et al. A novel microRNA-124/PTPN1 signal pathway mediates synaptic and memory deficits in Alzheimer′s disease [J]. Biol Psychiatry，2017. pii： S0006-3223（17）31853-X. doi： 10.1016/j.biopsych.2017.07.023. [Epub ahead of print]
[15] Chen JJ，Zhao B，Zhao J，et al. Potential roles of exosomal microRNAs as diagnostic biomarkers and therapeutic application in Alzheimer's disease [J]. Neural Plast，2017， 2017：7027380.
[16] Zhao J，Yue D，Zhou Y，et al. The role of microRNAs in Aβ deposition and tau phosphorylation in Alzheimer's disease [J]. Front Neurol，2017，8：342.
[17] Maoz R，Garfinkel BP，Soreq H. Alzheimer's Disease and ncRNAs [J]. Adv Exp Med Biol，2017，978：337-361.
[18] Prasad KN. Oxidative stress and pro-inflammatory cytokines may act as one of the signals for regulating microRNAs expression in Alzheimer's disease [J]. Mech Ageing Dev，2017，162：63-71.
[19] Basavaraju M，de Lencastre A. Alzheimer's disease：presence and role of microRNAs [J]. Biomol Concepts，2016， 7（4）：241-252.
[20] Batistela MS，Josviak ND，Sulzbach CD，et al. An overview of circulating cell-free microRNAs as putative biomarkers in Alzheimer's and Parkinson's Diseases [J]. Int J Neurosci，2017，127（6）：547-558.
2017, Vol. 14 
京公网安备 11010502046598号