Effects of testosterone in serum and follicular fluid on pregnancy outcomes of IVF-ET
LU Zhenhua1,2 HU Xingwen3▲ ZHENG Jie4 XIA Min4 XIA Qi3
1.Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China;
2.Hubei Provincial Institute of Traditional Chinese Medicine, Hubei Province, Wuhan 430074, China;
3.Department of Clinical Laboratory, Hubei Maternal and Child Health Hospital, Hubei Province, Wuhan 430070, China;
4.Reproductive Center of Hubei Maternal and Child Health Hospital, Hubei Province, Wuhan 430070, China
Abstract：Objective To investigate the effects of estradiol, progesterone and testosterone in serum and follicular fluid on pregnancy outcomes of in vitro fertilization - embryo transfer (IVF-ET). Methods From January to December 2013, 176 patients who received IVF-ET in the Reproductive Center of Hubei Maternal and Child Health Hospital were selected. According to clinical pregnancy outcome, clinical pregnancy group and non-clinical pregnancy group. The levels of estradiol, progesterone and testosterone concrtilization-embryo ttansfer, estradiol, progesterone and testosterone of daily basal serum, HCG daily serum, the serum on the day of ovulation and follicular fluid of two groups were detected. Results The levels of estradiol and testosterone in HCG daily serum in the clinical pregnancy group were higher than that in the non-clinical pregnancy group, and the differences were significant (P < 0.05). The concentration of testosterone in the clinical pregnancy group was higher than that in the non-clinical pregnancy group, and the difference was significant (P < 0.05). The concentration levels of estradiol, progesterone and testosterone in the serum on the day of ovulation were positively correlated with the concentration levels in the follicular fluid (r = 0.236, 0.216, 0.426, P < 0.01). Conclusion The testosterone in follicular fluid can promote the maturation of oocytes and facilitate the clinical pregnancy of IVF-ET.
[1] 胡兴文，王维鹏，秦柳.7种细胞因子对体外受精-胚胎移植妊娠结局的影响[J].临床检验杂志，2016.34（3):193-195.
[2] 郑君辉.超声监测卵泡与子宫内膜在不孕症中的应用[J].中国城乡企业卫生，2018，2（2）：92-93.
[3] 覃小菊，叶敏，李碧兰，等.经阴道超声在监测不孕症患者卵泡发育与子宫内膜变化中的应用[J].中国医学工程，2017，25（12）：76-78.
[4] Dubey AK，Wang HA，Duffy P，et al. The correlation between follicular measurements，oocyte morphology，and fertilization rates in an in vitro fertilization program [J]. Fertil Steril，1995，64（4）：787-790.
[5] Li Y，Li RQ，Ou SB，et al. Assotiation between the proportion of dominant follicles and oocyte developmental competence [J]. J Assist Reprod Genet，2014，31（12）：1599-1604.
[6] Nevet AL，Léveillé MC，Leader A，et al. Transcriptional characteristic of different sized follicles in relation on embryo transferability：potential role of hepatocyte growth facter sigalling[J]. Mol Hum Reprod，2016，22：475-484.
[7] Zhang T，Wu C，Aiao F，et al. Effect of distribution of different diameter follicles on HCG day on the outcome of IVF-ET/ICSI [J]. J Reprod Med，2017，26（8)：796-800.
[8] 王若琳，钱卫平，周亮.高龄对卵母细胞质量的影响[J].生殖与避孕，2016，36（9）：752-757.
[9] 高小琴，孙江华，袁佩华.体外受精－胚胎移植治疗中卵子和胚胎质量对生化妊娠周期的影响[J].中国妇幼保健，2018，33（2）：404-406.
[10] Iwata H. Age-associated changes in granulosa cells and follicular fluid in cows[J]. J Reprod Dev，2017，63（4）:339-345.
[11] 宋家美，陈慧，徐佩琦，等.女性基础睾酮水平对体外受精-胚胎移植妊娠结局的影响[J].昆明医科大学学报，2017，38（7）：93-98.
[12] 安锦霞，倪亚莉.雄激素在卵巢储备功能低下妇女中的研究进展[J].国际生殖健康/计划生育杂志，2013，32（1）：42-44
[13] 聂明月，方颖，张军，等.基础睾酮水平对卵巢储备功能下降患者体外受精-胚胎移植结局的预测[J].中国优生与遗传杂志，2015，23（3）:96-98.
[14] Bosdou JK，Venetis CA，Kolibianakis EM，et al. The use of androgens or androgen -modulating agents in poor responders undergoing in vitro fertilization：a systematic review and meta -analysis [J]. Hum Reprod Update，2012， 18（2）：127-145.
[15] Kim CH，Howles CM，Lee HA. The effect of transdermal testos-terone gel pretreatment on controlled ovarian stimulation and IVF outcome in low responders [J]. Fertil Steril，2011，95 （2）：679-683.
[16] 崔毓桂，邵丽，千日成，等．雄激素与女性生殖[J].国际生殖健康/计划生育杂志，2014，33（5）：329-333.
[17] Catteau-Jonard S，Dewailly D. Pathophysiology of polycystic ovary syndrome：the role of hyperandrogenism [J]. Front Horm Res，2013，40（1）：22-27.
[18] Lakshmi C. Hormone therapy in acne [J]. Indian J Dermatol Venereol Leprol，2013，79（3）：322-337.
[19] Mnif MF，Kamoun M，Kacem FH，et al. Reproductive outcomes of female patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency [J]. Indian J Endocrinol Metab，2013，17（5）：790-793.
[20] 刘娟，曾玉燕，王小云，等.HCG日孕酮水平与体外受精-胚胎移植治疗结局的关系探讨[J].暨南大学学报：自然科学与医学版，2017，38（2）：167-171.
[21] 胡建新，孙兆林，何坚，等.睾酮对体外培养小鼠精原干细胞增殖与分化的影响[J].临床泌尿外科杂志，2009， 24（6）：460-467.
2019, Vol. 16 
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