Effect of Ningdong Granules on metabolism of dopaminergic neurons
LI Mengying1 JIANG Liyuan1 SUN Kexing2 Xiaohong LI2 LI Jijun2▲
1.Quzhou College of Technology, Zhejiang Province, Quzhou 324000, China;
2.Department of Integrated Traditional Chinese and Western Medicine, Shanghai Children′s Medical Center, Shanghai Jiaotong University, Shanghai 200127, China
Abstract：Objective To study the effect of Ningdong Granules on the metabolism of dopaminergic (DA) neurons. Methods Twelve 18-days-old fetuses were randomly divided into Ningdong Granules (NDG) group, control (CTR) group, haloperidol (Hal) group and normal (Normal) group, with 3 rats in each group. DA neurons of each group were separately isolated and cultured, and tyrosine hydroxylase (TH) anti-staining was performed on DA neuron cells by immunofluorescence indirect method. Neuron axon anti-Tau1 anti-labeling combination with FITC fluorescence (green light) Ⅱ anti-labeling, neuron axon anti-Tau1 labeling combined with Cy 3 fluorescence (red light) Ⅱ anti-labeling, DA neuron cells and neuron axons were identified by fluorescent staining. The contents of MAO, DA, 5-HT, HVA and 5-HIAA in DA neuron cell culture medium of 18-days-old fetuses before and 1, 6, 12 and 24 h after drug intervention were detected by ELISA, and the metabolism of metacells of DA energetic spirit in each group was recorded. Results Compared with 0 h, the contents of MAO, DA, 5-HT and HVA in the cell culture medium of NDG group increased significantly after 6 and 12 h of intervention (P < 0.05); compared with CTR group at the same time, there was no significant difference in the contents of MAO, DA, 5-HT and HVA in the cell culture medium of Hal group after 0-24 h of intervention (P > 0.05); after 0-24 h of intervention, the 5-HIAA content in cell culture of fetuses in each group had no significant difference (P > 0.05). Conclusion NDG can promote the metabolism of DA neurons, but has no significant effect on the metabolism of 5-HT.
2019, Vol. 16 
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