Effects of amentoflame on proliferation and proliferation-related protein expression of neuroblastoma cells in mice
ZHU Pingping1 YUAN Wei2 WANG Pengzhen3
1.Department of Neurology, Guangzhou Red Cross Hospital of Jinan University, Guangdong Province, Guangzhou 510220, China; 2.Department of Surgery, Guangzhou Red Cross Hospital of Jinan University, Guangdong Province, Guangzhou 510220, China; 3.Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital of Jinan University, Guangdong Province, Guangzhou 510220, China
Abstract：Objective To investigate the effects of amentoflame (AF) on proliferation and proliferation-related protein expression of neuroblastoma cells (Neuro-2a) in mice. Methods Neuro-2a cells were resuscitated, cultured, and selected for this study. The CCK-8 assay was divided into five groups: 0, 25, 50, 75 and 125 μmol/L groups, the cells were treated for 24 h. The effect of AF (75 μmol/L AF) on the percentage of G1 phase and S phase in the cell cycle of Neuro-2a was determined by cytometry. The effects of AF (75 μmol/L AF group) on the expression of proliferation-related mRNA and proteins in Neuro- 2a cells were detected by fluorescence quantitative PCR and Western blot. Results The results of CCK-8 showed that 25 and 50 μmol/L AF group had no significant effect on the activity of Neuro-2a cells (P＞0.05), while 75 μmol/L group AF significantly decreased the activity of Neuro-2a cells, and the difference was statistically significant (P＜0.05). Compared with the control group, the percentage of Neu-2A cells in G1 phase in 75 μmol/L AF group was significantly increased, and the difference was statistically significant (P＜0.05); compared with the control group, the percentage of S-phase cells of Neuro-2a cells in 75 μmol/L AF group was significantly decreased, and the difference was statistically significant (P＜0.05); however, 75 μmol/L AF group had no significant effect on the cell distribution in G2 phase of Neuro-2a cells. Quantitative PCR and Western blot results showed that 75 μmol/L AF inhibited the expression of β-catenin, CyclinD1, CyclinE, and Survivin mRNA and protein in Neuro-2a cells, and promote expression of p21 and p16 mRNA and protein (P＜0.05). Conclusion 75 μmol/L AF inhibited the proliferation of Neuro-2a cells by inhibiting the expression of β-catenin, CyclinD1, CyclinE, and Survivin, promoting the expression of proliferation-related proteins such as p21 and p16.
祝萍萍1 苑伟2 王鹏珍3. 穗花杉双黄酮对小鼠神经母细胞瘤细胞增殖及增殖相关蛋白表达的影响[J]. 中国医药导报, 2023, 20(36): 16-20.
ZHU Pingping1 YUAN Wei2 WANG Pengzhen3. Effects of amentoflame on proliferation and proliferation-related protein expression of neuroblastoma cells in mice. 中国医药导报, 2023, 20(36): 16-20.
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