Effects of Mashe Anjin Pills on airway inflammation and expression levels of mucin 5AC and γ-aminobutyric acid A receptor α subunit in rat model with hypersecretion of airway mucus in chronic obstructive pulmonary disease
SHANG Yanhua DENG Jinghua ZHANG Xiaoyang MA Zhen WANG Yudong
Department of Pulmonology, Hengshui Hospital of Traditional Chinese Medicine, Hebei Province, Hengshui 053000, China
Abstract：Objective To investigate the effects of Mashe Anjin Pills on airway inflammation and the expression levels of mucin 5AC (MUC5AC) and γ-aminobutyric acid A receptor α subunit (GABAARα) in rat model with hypersecretion of airway mucus in chronic obstructive pulmonary disease (COPD). Methods A total of 102 7-week-old male SD rats with body weight of (195±20) g were divided into normal group, model group, Ambroxol positive control group, and Chinese medicine low-dose, medium-dose, high-dose groups by random number table method, with 17 rats in each group. Except for the normal group, the other groups were treated with lipopolysaccharide drops combined with cigarette smoke exposure to establish COPD airway mucus hypersecretion rat model. After successful modeling, Ambroxol positive control group was given Ambroxol solution (54 mg/[kg·d]), Chinese medicine low-dose, medium-dose, high-dose groups were given 4.375, 8.750, and 17.500 g/(kg·d) Mashe Anjin Pills (decoction), respectively, normal group and model group were given equal volume of normal saline, and all six groups were given continuous gavage for 28 days. After the experiment, hematoxylin-eosin staining and periodic acid Schiff stain were used to observe the pathological changes of lung tissue in each group. Serum inflammatory factors and alveolar lavage fluid cytokine levels were detected by enzyme-linked immunosorbent assay. The contents of MUC5AC and GABAARα in lung tissue were detected by Western blot. Results The pathological changes of airway and lung tissues in the model group were more serious. The levels of interleukin-1β (IL-1β), neutrophil elastase (NE), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β1), tissue metalloproteinase-1 (TIMP-1), matrix metalloproteinase-9 (MMP-9), MUC5AC, GABAARα of model group were higher than those of normal group, the differences were statistically significant (P＜0.05). The pathological changes of airway and lung tissues in Chinese medicine low-dose, medium-dose, high-dose groups were less than those in model group, and the levels of IL-1β, NE, TNF-α, TGF-β1, TIMP-1, MMP-9, MUC5AC, and GABAARα were lower than those in model group, the differences were statistically significant (P＜0.05). The levels of IL-1β, NE, MMP-9, and GABAARα in Chinese medicine medium-dose group were lower than those in Chinese medicine low-dose group. The levels of IL-1β, NE, TNF-α, TGF-β1, TIMP-1, MMP-9, MUC5AC, and GABAARα in Chinese medicine high-dose group were lower than those in Chinese medicine low-dose and medium-dose groups, and the differences were statistically significant (P＜0.05). Conclusion Mashe Anjin Pills can improve airway mucus hypersecretion and inhibit airway remodeling in COPD, which may be related to reducing inflammatory injury and inhibiting the expression of MUC5AC and GABAARα.
尚艳花 邓敬华 张晓阳 马真 王玉栋. 麻射安金丸对慢性阻塞性肺疾病气道黏液高分泌大鼠模型气道炎症及黏蛋白5AC和γ-氨基丁酸A受体α亚基表达水平的影响[J]. 中国医药导报, 2023, 20(36): 10-15.
SHANG Yanhua DENG Jinghua ZHANG Xiaoyang MA Zhen WANG Yudong. Effects of Mashe Anjin Pills on airway inflammation and expression levels of mucin 5AC and γ-aminobutyric acid A receptor α subunit in rat model with hypersecretion of airway mucus in chronic obstructive pulmonary disease. 中国医药导报, 2023, 20(36): 10-15.
2023, Vol. 20 
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