基于代谢组学探讨痹症宁方治疗风寒湿痹型类风湿关节炎的作用机制

doi:10.20047/j.issn1673-7210.2023.34.04

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摘要目的基于血清代谢组学的方法探究痹症宁方抗风寒湿痹型类风湿关节炎的作用机制。方法将24只7周龄160~180 g的SPF级Wistar雌性大鼠根据随机数字表法分为正常组、模型组、痹症宁方组，每组8只。模型组、痹症宁方组大鼠制备风寒湿痹型类风湿关节炎大鼠模型，造模7 d后出现活动受限、右足明显肿胀，左侧也可见关节肿胀，偶有关节变形，提示造模成功。痹症宁方组给予痹症宁方17.86 g/（kg·d）干预28 d，正常组和模型组给予等量生理盐水，给药时间为28 d。观察大鼠毛发色泽、饮食、行走活动等一般情况变化，分析及筛选差异代谢物及代谢通路。结果模型组较正常组，大鼠精神状态萎靡、毛发欠缺光泽、饮食进水减少、行走时右足跛行明显；痹症宁方组较模型组大鼠精神状态、毛发光泽、饮食进水、行走时右足跛行情况改善。模型组较正常组大鼠滑膜组织增生明显，关节腔模糊、狭窄，且炎症细胞浸润明显，关节骨质破坏且关节面粗糙不平，血管翳形成；痹症宁方组较模型组大鼠滑膜组织增生减少，关节腔逐渐清晰、增大，且炎症细胞浸润明显减少，关节骨质破坏及关节面粗糙不平明显改善，血管翳数量明显减少。正常组与模型组得到差异代谢物有45种，模型组与痹症宁方组得到差异代谢物有51种；主要涉及的通路有类固醇激素生物合成、精氨酸和脯氨酸代谢等。结论痹症宁方可能通过影响精氨酸和脯氨酸代谢，卟啉和叶绿素代谢，甘氨酸、丝氨酸和苏氨酸代谢等发挥作用。

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	何东初1*　　李蒋凤2*

关键词 ：痹症宁方, 代谢组学, 风寒湿痹, 类风湿关节炎

Abstract：Objective To explore the action mechanism of Bizhengning Decoction against wind-cold-dampness bi type rheumatoid arthritis based on the method of serum metabolomics. Methods A total of 24 SPF Wistar female rats, 160-180 g at seven weeks were divided into normal group, model group, and Bizhengning Decoction group according to the random number table method, with eight rats in each group. Wind-cold-dampness bi type rheumatoid arthritis rat model was prepared in model group and Bizhengning Decoction group, after seven days of modeling, there was limited movement, obvious swelling of the right foot, swelling of the left joint, and deformation of the relevant joint, indicating successful modeling. Bizhengning Decoction group was given 17.86 g/（kg·d） intervention for 28 days, and normal group and model group were given the same amount of normal saline for 28 days. The changes of hair color, diet, walking activity, and other general conditions were observed; differential metabolites and metabolic pathways were analyzed and screened. Results Compared with normal group, rats in the model group had weak mental state, less glossy hair, less dietary water intake, and obvious lameness on the right foot when walking; compared with model group, the mental state, hair luster, eating water, and walking lameness of right foot were improved in Bizhengning Decoction group. Compared with the normal group, the synovial tissue was hyperplasia, the joint cavity was blurred and narrow, and the inflammatory cell infiltration was obvious, the joint bone was destroyed, the joint surface was rough and uneven, and pannus was formed in the rate of the model group; compared with model group, the synovial tissue proliferation decreased, the joint cavity became clear and enlarged gradually, the inflammatory cell infiltration decreased significantly, the joint bone destruction and the joint surface roughness improved significantly, and the number of pannus decreased significantly in Bizhengning Decoction group. There were 45 different metabolites in normal group and model group, and 51 different metabolites in model group and Bizhengning Decoction group; the main pathways involved steroid hormone biosynthesis, arginine and proline metabolism, etc. Conclusion Bizhengning Decoction may affects the metabolism of arginine and proline, porphyrin and chlorophyll, and glycine, serine and threonine, etc.

Key words： Bizhengning Decoction Metabolomics Wind-cold-dampness bi Rheumatoid arthritis

基金资助:湖北省卫生和计划生育委员会科研项目（鄂卫生计生通〔2017〕20号）。

作者简介: 何东初（1966.11-），男，博士，主任医师，博士生导师，中国人民解放军中部战区总医院中西医结合科主任；研究方向：中西医结合治疗免疫性疾病。李蒋凤（1995.11-），女，湖北中医药大学中医临床学院2020级中西医结合临床专业在读博士研究生；研究方向：中西医结合治疗免疫性疾病。 *具有同等贡献

引用本文:

何东初1*　　李蒋凤2*. 基于代谢组学探讨痹症宁方治疗风寒湿痹型类风湿关节炎的作用机制[J]. 中国医药导报, 2023, 20(34): 21-25,35.
HE Dongchu1* LI Jiangfeng2*. Explore the action mechanism of Bizhengning Decoction in the treatment of wind-cold-dampness bi type rheumatoid arthritis based on metabonomics. 中国医药导报, 2023, 20(34): 21-25,35.

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