Expression of miR-30a in gastrointestinal stromal tumors and its mechanism of regulating cell proliferation, invasion and apoptosis
REN Hua1 MA Ming1 WANG Chao1 WANG Zhaojun2
1.Department of General Surgery, the Fifth People’s Hospital of Datong, Shanxi Province, Datong 037000, China; 2.Department of Physiology, Shanxi Medical University, Shanxi Province, Jinzhong 030600, China
Abstract：Objective To observe the expression of microRNA-30a (miR-30a) in gastrointestinal stromal tumors (GIST) and explore its effects on cell biological behaviors and possible mechanisms. Methods Thirty patients with GIST who underwent surgical resection in the Fifth People’s Hospital of Datong from November 2021 to November 2022 were selected as the study object, and the normal tissues removed by surgery (＞ 5 cm away from the edge of GIST tissue) were taken as the control. The expression of miR-30a in GIST tissues and normal tissues was compared. The logarithmic phase GIST-T1 cell line was taken, and the cells transfected with miR-30a negative control plasmid miR-30a NC, overexpression plasmid miR-30a mimics, and silencing plasmid miR-30a inhibitor were set as miR-30a NC group, miR-30a mimics group, and miR-30a inhibitor group, respectively. The cells without treatment were taken as the control group. The proliferation, invasion and apoptosis of each group were detected. The expression of transforming growth factor β1 (TGF-β1), p-Smad2/Smad2 protein were detected. The target relationship between miR-30a and TGF-β1 was verified. Results Compared with normal tissues, the expression of miR-30a in GIST tissues was decreased (P＜0.05). There were no significant differences in proliferation rate at 24, 48 and 72 h, number of transmembrane stained cells, apoptosis rate, TGF-β1 protein expression, and P-Smad2 /Smad2 between miR-30a NC group and control group (P＞0.05). Compared with the control group, the proliferation rate of miR-30a mimics group at 24, 48, 72 h were decreased, the number of stained cells passing through the membrane was reduced, the apoptosis rate was increased, the proliferation rate of miR-30a inhibitor group at 24, 48, 72 h were increased, the number of stained cells passing through the membrane was increased, the apoptosis rate was decreased (P＜0.05). Compared with the control group, the expression of TGF-β1 protein and p-Smad2/Smad2 in miR-30a mimics group was decreased, while those in miR-30a inhibitor group were increased (P＜0.05). Dual luciferase assay showed that TGF-β1 was a target gene of miR-30a. Conclusion The expression of miR-30a in GIST is decreased, which may regulate cell proliferation, invasion and apoptosis by targeting TGF-β signaling pathway.
任华1 马明1 王超1 王昭君2. miR-30a在胃肠道间质瘤中的表达及其调控细胞增殖、侵袭和凋亡的机制[J]. 中国医药导报, 2023, 20(33): 16-21.
REN Hua1 MA Ming1 WANG Chao1 WANG Zhaojun2. Expression of miR-30a in gastrointestinal stromal tumors and its mechanism of regulating cell proliferation, invasion and apoptosis. 中国医药导报, 2023, 20(33): 16-21.
2023, Vol. 20 
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