miR-30a在胃肠道间质瘤中的表达及其调控细胞增殖、侵袭和凋亡的机制

doi:10.20047/j.issn1673-7210.2023.33.03

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摘要目的观察微RNA-30a（miR-30a）在胃肠道间质瘤（GIST）中的表达，并探讨其对细胞生物学行为的影响及可能机制。方法选取2021年11月至2022年11月在山西省大同市第五人民医院进行手术切除治疗的30例GIST患者作为研究对象，其中手术切除的正常组织（距离GIST组织边缘＞5 cm）作为对照。比较GIST组织及正常组织miR-30a表达量。取对数期GIST-T1细胞株，将转染miR-30a阴性对照质粒miR-30a NC、过表达量质粒miR-30a mimics、沉默质粒miR-30a inhibitor的细胞分别设为miR-30a NC组、miR-30a mimics组、miR-30a inhibitor组，取不作处理的细胞为对照组。检测各组增殖、侵袭及凋亡情况；检测细胞转化生长因子β1（TGF-β1）、p-Smad2/Smad2蛋白表达量；验证miR-30a与TGF-β1的靶向关系。结果与正常组织比较，GIST组织中的miR-30a表达量降低（P＜0.05）。miR-30a NC组24、48、72 h增殖率，穿膜染色细胞数，凋亡率，TGF-β1蛋白表达量，p-Smad2/Smad2与对照组比较，差异均无统计学意义（P＞0.05）。与正常组织比较，GIST组织中的miR-30a表达量降低（P＜0.05）。与对照组比较，miR-30a mimics组24、48、72 h细胞增殖率降低，穿膜染色细胞数减少，凋亡率升高（P＜0.05），miR-30a inhibitor组24、48、72 h细胞增殖率升高，穿膜染色细胞数增加，凋亡率降低（P＜0.05）。与对照组比较，miR-30a mimics组TGF-β1蛋白表达量、p-Smad2/Smad2降低，miR-30a inhibitor组TGF-β1蛋白表达量、p-Smad2/Smad2升高（P＜0.05）。双萤光素酶实验显示，TGF-β1是miR-30a的一个靶基因。结论 miR-30a在GIST中表达降低，可能通过靶向抑制TGF-β信号通路活性影响细胞增殖、侵袭和凋亡。

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	任华1　　马明1　　王超1　　王昭君2

关键词 ：胃肠道间质瘤, 微RNA-30a, 增殖, 侵袭, 凋亡

Abstract：Objective To observe the expression of microRNA-30a (miR-30a) in gastrointestinal stromal tumors (GIST) and explore its effects on cell biological behaviors and possible mechanisms. Methods Thirty patients with GIST who underwent surgical resection in the Fifth People’s Hospital of Datong from November 2021 to November 2022 were selected as the study object, and the normal tissues removed by surgery (＞ 5 cm away from the edge of GIST tissue) were taken as the control. The expression of miR-30a in GIST tissues and normal tissues was compared. The logarithmic phase GIST-T1 cell line was taken, and the cells transfected with miR-30a negative control plasmid miR-30a NC, overexpression plasmid miR-30a mimics, and silencing plasmid miR-30a inhibitor were set as miR-30a NC group, miR-30a mimics group, and miR-30a inhibitor group, respectively. The cells without treatment were taken as the control group. The proliferation, invasion and apoptosis of each group were detected. The expression of transforming growth factor β1 (TGF-β1), p-Smad2/Smad2 protein were detected. The target relationship between miR-30a and TGF-β1 was verified. Results Compared with normal tissues, the expression of miR-30a in GIST tissues was decreased (P＜0.05). There were no significant differences in proliferation rate at 24, 48 and 72 h, number of transmembrane stained cells, apoptosis rate, TGF-β1 protein expression, and P-Smad2 /Smad2 between miR-30a NC group and control group (P＞0.05). Compared with the control group, the proliferation rate of miR-30a mimics group at 24, 48, 72 h were decreased, the number of stained cells passing through the membrane was reduced, the apoptosis rate was increased, the proliferation rate of miR-30a inhibitor group at 24, 48, 72 h were increased, the number of stained cells passing through the membrane was increased, the apoptosis rate was decreased (P＜0.05). Compared with the control group, the expression of TGF-β1 protein and p-Smad2/Smad2 in miR-30a mimics group was decreased, while those in miR-30a inhibitor group were increased (P＜0.05). Dual luciferase assay showed that TGF-β1 was a target gene of miR-30a. Conclusion The expression of miR-30a in GIST is decreased, which may regulate cell proliferation, invasion and apoptosis by targeting TGF-β signaling pathway.

Key words： Gastrointestinal stromal tumor MicroRNA-30a Proliferation Invasion Apoptosis

基金资助:山西省自然科学研究面上项目（20210302123306）。

引用本文:

任华1　　马明1　　王超1　　王昭君2. miR-30a在胃肠道间质瘤中的表达及其调控细胞增殖、侵袭和凋亡的机制[J]. 中国医药导报, 2023, 20(33): 16-21.
REN Hua1 MA Ming1 WANG Chao1 WANG Zhaojun2. Expression of miR-30a in gastrointestinal stromal tumors and its mechanism of regulating cell proliferation, invasion and apoptosis. 中国医药导报, 2023, 20(33): 16-21.

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