Effect of six-segment transmembrane epithelial antigen of prostate 4 on proliferation of prostate cancer cells in inflammatory environment
SUN Xinghua1 YU Tao2 ZHANG Xuexin3 CHANG Hongyan1 HONG Yueguang1 LI Weiwei4
1.Department of Oncology, Qinhuangdao Hospital of Traditional Chinese Medicine, Hebei Province, Qinhuangdao 066000, China; 2.College of Life Sciences and Technology, Weifang Medical University, Shandong Province, Weifang 261053, China; 3.Department of Cardiovascular Medicine, Qinhuangdao Hospital of Traditional Chinese Medicine, Hebei Province, Qinhuangdao 066000, China; 4.Department of Reproductive Medicine, Qinhuangdao Maternal and Child Health Care Hospital, Hebei Province, Qinhuangdao 066000, China
Abstract：Objective To investigate the role of six-segment transmembrane epithelial antigen of prostate 4 (STEAP4) in the development of prostate cancer in the inflammatory microenvironment induced by lipopolysaccharide (LPS). Methods The inflammatory environment of prostate cancer cells PC3 and VCaP was induced by LPS. PC3 and VCaP cells were divided into control group, LPS treated group (PC3 and VCaP cells were exposed to 1 μg/ml LPS), transfection control group(LPS+transfection si-con) and transfection silence group (LPS+transfection si-STEAP4). The STEAP4 level was detected 24 h after transfection by Western blot. The expression levels of cytokines interleukin (IL)-6, IL-8 and tumor necrosis factor-α(TNF-α) were detected by enzyme-linked immunosorbent assay. Cell proliferation was detected by CCK-8 and EdU staining. The expression level of cyclic guanosine monophosphate(cGMP)-cGMP-dependent protein kinase(PKG) signaling pathway related proteins was detected by Western blot. Results STEAP4 protein expression level of PC3 and VCaP cells in LPS treated group was significantly higher than that in control group (P＜0.05). There was no significant difference in the expression level of STEAP4 protein in PC3 and VCaP cells in LPS treated group compared with transfection control group (P＞0.05). The expression level of STEAP4 protein in PC3 and VCaP cells in transfection silence group was significantly lower than that in transfection control group (P＜0.05). The expression levels of IL-6, IL-8, and TNF-α in PC3 and VCaP cells in LPS treated group were significantly higher than those in control group (P＜0.05). There were no significant differences in the expression levels of IL-6, IL-8, and TNF-α in PC3 and VCaP cells between LPS treated group and transfection control group (P＞0.05). The expression levels of IL-6, IL-8, and TNF-α in PC3 and VCaP cells in transfection silence group were significantly lower than those in transfection control group (P＜0.05). The proliferation activity of PC3 and VCaP cells in LPS treated group was significantly higher than that in control group at 24, 48, and 72 h (P＜0.05). There was no significant difference in the proliferation activity of PC3 and VCaP cells at 24, 48, and 72 h between LPS treated group and transfection control group (P＞0.05). The proliferation activity of PC3 and VCaP cells in transfection silence group was lower than that in transfection control group at 24, 48, and 72 h (P＜0.05). The proliferation activity of PC3 and VCaP cells in LPS treated group was significantly higher than that in control group (P＜0.05). There was no significant difference in the proliferation activity of PC3 and VCaP cells between LPS treated group and transfection control group (P＞0.05). The proliferation activity of PC3 and VCaP cells in transfection silence group was significantly lower than that in transfection control group (P＜0.05). The relative expression levels of cGMP, PKG1, PKG2, and phosphorylated vasodilator stimulated phosphoprotein (pVASP)/VASP in PC3 and VCaP cells of LPS treated group were significantly lower than those of control group (P＜0.05). There were no significant differences in the relative expression levels of cGMP, PKG1, PKG2, and pVASP/VASP in PC3 and VCaP cells between transfection control group and LPS treated group (P＞0.05). The relative expression levels of cGMP, PKG1, PKG2, and pVASP/VASP in PC3 and VCaP cells of transfection silence group were significantly higher than those of transfection control group (P＜0.05). Conclusion STEAP4 silencing inhibits the proliferation and inflammatory response of prostate cancer cells induced by LPS. Downregulation of STEAP4 may inhibit LPS-induced tumorigenesis by reducing cell proliferation and inflammatory response.
孙兴华1 于涛2 张学新3 常鸿艳1 洪月光1 李伟伟4. 前列腺六段跨膜上皮抗原4对炎症环境下前列腺癌细胞增殖的影响[J]. 中国医药导报, 2023, 20(33): 4-10.
SUN Xinghua1 YU Tao2 ZHANG Xuexin3 CHANG Hongyan1 HONG Yueguang1 LI Weiwei4. Effect of six-segment transmembrane epithelial antigen of prostate 4 on proliferation of prostate cancer cells in inflammatory environment. 中国医药导报, 2023, 20(33): 4-10.
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