Expression of lncRNA XIST and miR-132-3p in endometrial cancer tissues and the relationship with pathological parameters and prognosis
REN Xiaoxia1 XU Haibo2 WU Jinting3 WANG Chenyi4 HAN Yaqin1
1.Department of Obstetrics and Gynecology, the Sixth People’s Hospital of Nantong Affiliated Nantong Hospital of Shanghai University, Jiangsu Province, Nantong 226000, China; 2.Department of Gynecology, Nantong Tumor Hospital, Jiangsu Province, Nantong 226000, China; 3.Department of Gynecology, Nantong Maternal and Child Health Care Hospital, Jiangsu Province, Nantong 226000, China; 4.Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Jiangsu Province, Nantong 226000, China
Abstract：Objective To investigate the expression of long non-coding RNA X inactive specific transcript (lncRNA XIST), microRNA-132-3p (miR-132-3p) in endometrial cancer (EC) tissues and the relationship with pathological parameters and prognosis. Methods A total of 100 EC patients admitted to the Sixth People’s Hospital of Nantong and Nantong Tumor Hospital, Jiangsu Province from February 2016 to December 2019 were selected. qPCR was used to detect the expression of lncRNA XIST and miR-132-3p in cancer and paracancer tissues. The StarBase database predicted the relationship between lncRNA XIST and miR-132-3p, and Pearson correlation analysis was used to correlate the expression of lncRNA XIST and miR-132-3p. At three years follow-up, according to the average expression of lncRNA XIST and miR-132-3p in EC tissues, the patients were divided into high expression group and low expression group, and the survival curves of patients with different lncRNA XIST and miR-132-3p expression in EC tissues were plotted using the K-M method. Results lncRNA XIST expression in cancer tissue was higher than that in paracancerous normal tissue, and miR-132-3p expression was lower than that in paracancerous normal tissue (P＜0.05). There was a complementary sequence between lncRNA XIST and miR-132-3p, lncRNA XIST was negatively correlated with miR-132-3p expression in EC tissues (r =-0.694, P＜0.01). The expression of lncRNA XIST and miR-132-3p in EC tissues of different differentiation degrees, International Federation of Obstetrics and Gynecology (FIGO) stage, vascular invasion, and lymph node metastasis were statistically significant (P＜0.05). The 3-year cumulative survival rate of the lncRNA XIST high expression group was lower than that of the lncRNA XIST low expression group, and the 3-year cumulative survival rate of the miR-132-3p high expression group was higher than that of the miR-132-3p low expression group (P＜0.05). Conclusion The expression of lncRNA XIST is high and that of miR-132-3p is low in EC tissue, and its correlate with differentiation degree, FIGO stage, vascular invasion, and lymph node metastasis and prognosis.
任小霞1 徐海波2 吴金婷3 王晨毅4 韩亚琴1. 子宫内膜癌组织中lncRNA XIST、miR-132-3p的表达及与病理参数和预后的关系[J]. 中国医药导报, 2023, 20(32): 33-37.
REN Xiaoxia1 XU Haibo2 WU Jinting3 WANG Chenyi4 HAN Yaqin1. Expression of lncRNA XIST and miR-132-3p in endometrial cancer tissues and the relationship with pathological parameters and prognosis. 中国医药导报, 2023, 20(32): 33-37.
[1] Sung H，Ferlay J，Siegel RL，et al. Global cancer statistics 2020：globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin，2021，71（3）：209-249. [2] Xia C，Dong X，Li H，et al. Cancer statistics in China and United States，2022：profiles，trends，and determinants [J]. Chin Med J（Engl），2022，135（5）：584-590. [3] 中国抗癌协会妇科肿瘤专业委员会.子宫内膜癌诊断与治疗指南（2021年版）[J].中国癌症杂志，2021，31（6）：501-512. [4] 李文龙，林晓雨，李小文，等.子宫内膜癌中的lncRNA- miRNA-mRNA ceRNA网络的整合分析[J].生命的化学，2021，41（2）：379-386. [5] 陈洁，洪于贺，尹乐乐，等.外泌体源miRNA在子宫内膜癌中的研究进展[J].中国计划生育和妇产科，2022，14（5）：43-46. [6] Liu JH，Li C，Cao L，et al. Cucurbitacin B regulates lung cancer cell proliferation and apoptosis via inhibiting the IL-6/ STAT3 pathway through the lncRNA XIST/miR-let-7c axis [J]. Pharm Biol，2022，60（1）：154-162. [7] 范渊，吴海龙，竹梦，等.lncRNA XIST通过靶向miR-3666调控胃癌细胞增殖、侵袭转移机制[J].中国免疫学杂志，2022，38（8）：964-968. [8] 李昌伟，张浩，余壮明，等.miR-132-3p靶向抑制FOXP2基因表达对乳腺癌细胞增殖、侵袭和迁移的影响[J].中国老年学杂志，2021，41（9）：1903-1907. [9] Huang R，Xiang G，Duan X，et al. MiR-132-3p inhibits proliferation，invasion and migration of colorectal cancer cells via down-regulating FOXP2 expression [J]. Acta Biochim Pol，2022，69（2）：371-377. [10] Pecorelli S. Revised FIGO staging for carcinoma of the vulva，cervix，and endometrium [J]. Int J Gynaecol Obstet，2009， 105（2）：103-104. [11] 中国医师协会微无创医学专业委员会妇科肿瘤学组，山东省抗癌协会妇科肿瘤分会.复发性子宫内膜癌诊治的中国专家共识（2022年版）[J].中华肿瘤防治杂志，2022，29（21）：1517-1527. [12] 中华医学会妇科肿瘤学分会.妇科恶性肿瘤紫杉类药物临床应用专家共识[J].现代妇产科进展，2019，28（10）：724-730. [13] 中华医学会妇科肿瘤学分会.妇科恶性肿瘤聚乙二醇化脂质体多柔比星临床应用专家共识[J].中国医学前沿杂志（电子版），2020，12（7）：24-32. [14] 邓波儿，孔为民.子宫内膜癌的表观遗传学研究进展[J].国际肿瘤学杂志，2021，48（3）：184-188. [15] Kong D，Hou Y，Li W，et al. LncRNA-ZXF1 stabilizes P21 expression in endometrioid endometrial carcinoma by inhibiting ubiquitination-mediated degradation and regulating the miR-378a-3p/PCDHA3 axis [J]. Mol Oncol，2022， 16（3）：813-829. [16] Chen Q，Schatz C，Cen Y，et al. LncRNA TUG1 promotes the migration and invasion in type Ⅰ endometrial carcinoma cells by regulating E-N cadherin switch [J]. Taiwan J Obstet Gynecol，2022，61（5）：780-787. [17] 陈坚，肖宗位，高珂，等.LncRNA XIST靶向miR-511-3p调控食管癌细胞增殖、迁移和侵袭的机制研究[J].河北医药，2022，44（6）：843-846，851. [18] 张璇，马里程，孙龙啸，等.干扰lncRNA XIST通过上调miR-429抑制涎腺腺样囊性癌细胞生长并提高顺铂敏感性[J].新医学，2022，53（7）：515-523. [19] Chen Y. Identification and validation of cuproptosis-related prognostic signature and associated regulatory axis in ute- rine corpus endometrial carcinoma [J]. Front Genet，2022，7（13）：912037. [20] 王媛媛，宋成文，陈滢，等.lncRNA XIST/miR-153/SNAI1在宫颈癌迁移、侵袭和上皮间质转化中的表达及意义[J].中国妇产科临床杂志，2022，23（5）：470-473. [21] 周新朋，金晔华，张润润，等.类风湿关节炎成纤维样滑膜细胞lncRNA-miRNA-mRNA网络构建及关键基因预测[J].中国医药导报，2021，18（9）：12-16，封3. [22] 马守叶，赵晶，魏振宏.微小RNA与子宫内膜癌的相关性[J].国际妇产科学杂志，2022，49（4）：371-375. [23] 汪根良，华崇俊，张向明，等.miR-132-3p靶向调控Gab2抑制胃癌细胞增殖、迁移及侵袭分子机制的研究[J].世界华人消化杂志，2020，28（1）：9-17. [24] 刘颖，赵庆丽，乔如丽，等.miR-132-3p靶向负调控PSMD12抑制乳腺癌上皮间质转化[J].现代肿瘤医学，2021，29（17）：2963-2970. [25] 徐洪阁，丁红岩，张婷.过表达miR-132-3p对子宫内膜癌细胞增殖、凋亡、侵袭及迁移的作用[J].诊断病理学杂志，2021，28（7）：546-551. [26] 鲍慧，孙晓东，乔健，等.LncRNA XIST靶向miR-132-3p影响人宫颈癌细胞株Hela增殖、侵袭和迁移[J].微循环学杂志，2022，32（4）：1-8.
2023, Vol. 20 
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