电针预处理对心肌缺血再灌注损伤大鼠线粒体自噬相关蛋白表达的影响

doi:10.20047/j.issn1673-7210.2023.31.05

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摘要目的研究电针预处理对心肌缺血再灌注损伤大鼠线粒体自噬相关蛋白表达的影响。方法将36只雄性SD大鼠，8～10周龄，体重180～220 g，采用随机数字表法将其分为对照组、模型组和电针预处理组，各12只。再利用离体灌流系统制备心肌缺血再灌注损伤大鼠模型，对照组大鼠只进行平衡灌注3 h，模型组与电针预处理组大鼠平衡灌注20 min后，缺血40 min，再灌注2 h，造模前将电针预处理组用电针干预3周。取心肌组织检测肌酸激酶（CK）、肌酸激酶同工酶MB（CK-MB）、乳酸脱氢酶（LDH）含量；苏木精-伊红（HE）染色观察各组大鼠心肌细胞中的病理变化；Western blot法检测线粒体自噬相关蛋白Beclin-1、P62、PINK1与Parkin表达。结果模型组CK、CK-MB、LDH含量高于对照组，差异有统计学意义（P＜0.05）；电针预处理组CK、CK-MB、LDH含量低于模型组，差异有统计学意义（P＜0.05）。HE染色结果显示：模型组心肌纤维多断裂、变性、坏死，且排列松散、不规则。电针预处理组心肌组织结构基本完整，胞间轻度水肿，肌纤维间隙轻度增宽，坏死灶减轻。模型组线粒体自噬标志蛋白Beclin-1、P62、PINK1、Parkin水平高于对照组，差异有统计学意义（P＜0.05）；电针预处理组线粒体自噬标志蛋白Beclin-1、PINK1、Parkin水平高于模型组，P62水平低于模型组，差异有统计学意义（P＜0.05）。结论电针预处理通过调控PINK1、Parkin蛋白表达，激活线粒体自噬来改善心肌缺血再灌注损伤，发挥心肌保护作用。

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	唐强1　　马西元1　　刘欣欣1　　尹侠1　　赵耀伟1　　李宇婷1　　孟享泓1　　李宏玉2▲

关键词 ：电针, 线粒体自噬, PINK1, Parkin, 心肌缺血再灌注

Abstract：Objective To investigate the effect of electroacupuncture preconditioning on the expression of autophagy-related proteins in myocardial ischemia-reperfusion injury rats’ mitochondria. Methods Thirty-six male SD rats, aged 8-10 weeks and with body weight 180-220 g were divided into control group, model group, and electroacupuncture pretreatment group by random number table method. The rat myocardial ischemia-reperfusion injury model was developed by an outside-in perfusion system. Rats in control group were only perfused for three hours. Rats in model group and electroacupuncture pretreatment group were perfused for 20 minutes，then ischemia for 40 minutes, and reperfusion for two hours. The electroacupuncture pretreatment group underwent electroacupuncture treatment for three weeks before modeling. The contents of creatine kinase (CK), creatine kinase (CK-MB), and lactate dehydrogenase (LDH) were detected in myocardial tissue; hematoxylin-eosin (HE) staining was stained to visualize the lesions in the groups of cardiomyocytes. Mitochondrial autophagy-related proteins Beclin-1, P62, PINK1, and Parkin exposure were identified by Western blot. Results The contents of CK, CK-MB, and LDH in the model group were higher than those in the control group, with statistical significances (P＜0.05); the contents of CK，CK-MB, and LDH in the electroacupuncture pretreatment group were less than those in the model group, with statistical significances (P＜0.05). HE staining results indicated that cardiac muscle fibers in the model group were mostly fractured, degenerated, necrotic, and arranged loosely and irregularly.The myocardial tissue structure in the electroacupuncture pretreatment group was basically intact, the intercellular edema was mild, the muscle fiber space was slightly widened, and the necrotic focus was lightened. The levels of mitochondrial autophagy marker proteins Beclin-1, P62, PINK1, and Parkin in model group were higher than those in control group, with statistical significances (P＜0.05). The levels of mitochondrial autophagy marker proteins Beclin-1, PINK1, and Parkin in electroacupuncture pretreatment group were higher than those in model group, and the level of P62 was lower than those in model group, with statistical significances (P＜0.05). Conclusion Electroacupuncture pretreatment can improve myocardial ischemia/reperfusion injury by regulating the expression of PINK1 and Parkin protein, and activating mitochondrial autophagy.

Key words： Electroacupuncture Mitophagy PINK1 Parkin Myocardialischemia-reperfusion

基金资助:黑龙江省中医药科研项目（ZHY2022-164）；
黑龙江省博士后基金项目（LBH-Z20201）。

通讯作者: ▲通讯作者

作者简介: 唐强（1963-），男，医学博士，主任医师，教授，博士生导师；研究方向：功能障碍的中医康复基础与临床研究。

引用本文:

唐强1　　马西元1　　刘欣欣1　　尹侠1　　赵耀伟1　　李宇婷1　　孟享泓1　　李宏玉2▲. 电针预处理对心肌缺血再灌注损伤大鼠线粒体自噬相关蛋白表达的影响[J]. 中国医药导报, 2023, 20(31): 30-33.
TANG Qiang1 MA Xiyuan1 LIU Xinxin1 YIN Xia1 ZHAO Yaowei1 LI Yuting1 MENG Xianghong1 LI Hongyu2▲. Effect of electroacupuncture preconditioning on the expression of mitop-hagy related proteins in myocardial ischemia-reperfusion injury rats. 中国医药导报, 2023, 20(31): 30-33.

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