Effects of acupuncture on the content of hydrogen sulfide and the expression of cystathionine-β-synthase and cystathione-γ lyase in the lungs of rats with chronic obstructive pulmonary disease
LIU Wenmei1 ZHANG Xinfang2 JIANG Chuanwei2 WANG Yuan2 XIANG Shuiying1 LIU Zibing1
1.College of Acupuncture, Moxibustion, and Massage, Anhui University of Chinese Medicine, Anhui Province, Hefei 230012, China; 2.College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Anhui Province, Hefei 230012, China
Abstract：Objective To observe the effects of acupuncture on the content of hydrogen sulfide (H2S) and the expression of cystathionine-β-synthase (CBS) and cystathionine-γ lyase (CSE) in lung tissue of chronic obstructive pulmonary disease (COPD) rats. Methods A total of 24 6-week-old clean grade male SD rats with body weight of (200±10) g were selected, and divided into control group, model group, and electroacupuncture group by random number table method, with eight rats in each group. The control group was fed conventionally. The COPD model was established by cigarette smoke combined with lipopolysaccharide in model group and electroacupuncture group. After successful modeling, the electroacupuncture group was treated with electricacupuncture on both fèishù and zúsānlǐ for two consecutive weeks, while the model group and control group were not treated. After the intervention, forced vital capacity (FVC), forced expiratory volume in 0.1 second (FEV0.1), FEV0.3, and peak expiratory flow (PEF) were measured. The pathological changes in the lungs of the three groups were observed. The levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in lung tissue and plasma of the three groups were detected by enzyme-linked immunosorbent assay. The contents of H2S, CBS, and CSE in lung tissue were detected. The correlation between IL-1β and TNF-α in lung tissue and H2S was further analyzed. Results FEV0.1, FEV0.3, FEV0.1/FVC, FEV0.3/FVC, and PEF in the model group were lower than those in the control group, and the differences were statistically significant (P＜0.05). Its pulmonary pathological results showed inflammatory cell infiltration and pulmonary interstitial thickening. IL-1β and TNF-α in lung tissue and plasma in model group were higher than those in control group, while H2S, CBS, CSE contents, and CBS, CSE protein expressions in lung tissue were higher than those in control group, and the differences were highly statistically significant (P＜0.01). FEV0.1, FEV0.3, FEV0.1/FVC, FEV0.3/FVC, and PEF in electroacupuncture group were higher than those in model group, and the differences were statistically significant (P＜0.05). IL-1β and TNF-α in lung tissue and plasma in the electroacupuncture group were lower than those in the model group, while the H2S, CBS, CSE contents and the CBS and CSE protein expression in lung tissue were lower than those in the model group, the differences were statistically significant (P＜0.05). Correlation analysis showed that IL-1β, TNF-α, and H2S in lung tissue were positively correlated (r＞0, P＜0.01). Conclusion Electroacupuncture in fèishù and zúsānlǐ can down-regulate the content and expression of H2S, CSE, and CBS in lung tissue of COPD rats, and H2S may be involved in the anti-infection effect of acupuncture.
刘文美1 张新芳2 江传玮2 王媛2 项水英1 刘自兵1. 针刺对慢性阻塞性肺疾病大鼠肺中硫化氢含量及胱硫醚β-合酶、胱硫醚γ裂解酶表达的影响[J]. 中国医药导报, 2023, 20(29): 25-28,38.
LIU Wenmei1 ZHANG Xinfang2 JIANG Chuanwei2 WANG Yuan2 XIANG Shuiying1 LIU Zibing1. Effects of acupuncture on the content of hydrogen sulfide and the expression of cystathionine-β-synthase and cystathione-γ lyase in the lungs of rats with chronic obstructive pulmonary disease. 中国医药导报, 2023, 20(29): 25-28,38.
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2023, Vol. 20 
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