Abstract：Objective To detect detection of small nucleolar RNA host gene 17 (SNHG17) How to regulate malignant phenotype of glioblastoma (GBM) by miR-23b-3p and zinc finger structure homeobox protein 1 (ZHX1). Methods Surgical specimens of 33 patients diagnosed with GBM in the Department of Neurosurgery, the Guangxi Zhuang Autonomous Region Brain Hospital from January 2020 to October 2022 were selected. The expression of SNHG17, miR-23b-3p, and ZHX1 in GBM samples and normal tissues adjacent to tumor were detected by real-time quantitative polymerase chain reaction (qRT-PCR). Pearson correlation analysis was used to evaluate the correlation between SNHG17, miR-23b-3p, and ZHX1. GBM cell line A172 and DK-MG were used as cell models, the cells were divided into si-NC group (transfected with blank inhibitor), si-SNHG17 group (transfected with si-SNHG17#2), and si-SNHG17+miR-23b-3p inhibitor group (transfected with si-SNHG17#2 and miR-23b-3p inhibitor). The proliferation capacity of GBM cells was detected by CCK-8 and EdU methods, while the migration and invasion of GBM cells were evaluated by Transwell assay. The qRT-PCR and Western blot were used to detect the mutual regulation of SNHG17 and miR-23b-3p, and their regulatory effect on ZHX1. Results The expression levels of SNHG17 and ZHX1 in GBM tissue were higher than those in normal tissue adjacent to cancer, while the expression level of miR-23b-3p was lower than that in normal tissue adjacent to cancer, the differences were statistically significant (P＜0.05). In GBM tissues, SNHG17 expression was negatively correlated with the expression of miR-23b-3p (r=-0.711, P＜0.05), but positively correlated with the expression of ZHX1 (r=0.648, P＜0.05); the expression of miR-23b-3p and ZHX1 was negatively correlated (r=-0.637, P＜0.05). The expression of miR-23b-3p in the si-SNHG17 group was higher than that in the si-NC group, and the proliferation, migration, and invasion ability of GBM cells and the expression level of ZHX1 were lower than those in the si-NC group, the differences were statistically significant (P＜0.05); however, the expression of miR-23b-3p in the si-SNHG17 + miR-23b-3p inhibitor group was lower than that in the si-SNHG17 group, and the proliferation, migration, invasion, and expression level of ZHX1 in GBM cells were higher than those in the si-SNHG17 group, the differences were statistically significant (P＜0.05). Conclusion SNHG17 can promote the proliferation, migration and invasion of GBM cells by regulating miR-23b-3p/ZHX1 axis. This suggests that SNHG17 may be a potential therapeutic target for GBM.
[1] ■ledzińska P，Bebyn MG，Furtak J，et al. Prognostic and Pred- ictive Biomarkers in Gliomas [J]. Int J Mol SCI，2021，22（19）： 10373.
[2] 张剑宁，刘聪为.脑胶质瘤新型治疗方案研究进展[J].肿瘤防治研究，2022，49（6）：9.
[3] Wang W，Yu S，Li W，et al. Silencing of lncRNA SNHG17 inhibits the tumorigenesis of epithelial ovarian cancer through regulation of miR-485-5p/AKT1 axis [J]. Biochem Biophys Res Commun，2022，637：117-126.
[4] Zhang G，Xu Y，Wang S，et al. LncRNA SNHG17 promotes gastric cancer progression by epigenetically silencing of p15 and p57 [J]. J Cell Physiol，2019，234（4）：5163-5174.
[5] 徐琛，徐辉甫，苏文.长链非编码RNA SNHG17通过微小RNA-375促进气道平滑肌细胞增殖和迁移[J].中国临床药理学杂志，2022，38（10）：1043-1046.
[6] Ma L，Gao J，Zhang N，et al. Long noncoding RNA SNHG17：a novel molecule in human cancers [J]. Cancer Cell Int，2022，22（1）：104.
[7] 李渊，王欣鑫，陈世勇，等.小核仁RNA宿主基因17在癌症进展中的作用研究进展[J].解放军医学杂志，2022，47（9）：932-940.
[8] Ma Z，Gu S，Song M，et al. Long non-coding RNA SNHG17 is an unfavourable prognostic factor and promotes cell proliferation by epigenetically silencing P57 in colorectal cancer [J]. Mol Biosyst，2017，13（11）：2350-2361.
[9] Hansen T，Jensen T，Clausen B，et al. Natural RNA circles function as efficient microRNA sponges [J]. Nature，2013， 495（7441）：384-388.
[10] Saliminejad K，Khorram Khorshid H，Soleymani Fard S，et al. An overview of microRNAs：Biology，functions，therapeutics，and analysis methods [J]. J Cell Physiol，2019，234（5）：5451-5465.
[11] Wang W，Zhang A，Hao Y，et al. The emerging role of miR-19 in glioma [J]. J Cell Mol Med，2018，22（10）：4611-4616.
[12] Park N，Cha J，Sung P，et al. MiR-23b-3p suppresses epithelial-mesenchymal transition，migration，and invasion of hepatocellular carcinoma cells by targeting c-MET [J]. Heliyon，2022，8：e11135.
[13] 熊先会，敖鸿舜，冯盈庭，等.miRNA在胃癌和肝癌中的研究进展[J].中国医药导报，2018，15（35）：30-33.
[14] Nawaz Z，Patil V，Paul Y，et al. PI3 kinase pathway regulated miRNome in glioblastoma：identification of miR-326 as a tumour suppressor miRNA [J]. Mol Cancer，2016，15（1）：74.
[15] Liu Y，Ma D，Ji C. Zinc fingers and homeoboxes family in human diseases [J]. Cancer Gene Ther，2015，22（5）：223-226.
[16] Liu G，Clement L，Kanwar Y，et al. ZHX proteins regulate podocyte gene expression during the development of nephrotic syndrome [J]. J Biol Inorg Chem，2006，281（51）：39681-39692.
[17] Nagel S，Ehrentraut S，Meyer C，et al. Aberrantly Expressed OTX Homeobox Genes Deregulate B-Cell Differentiation in Hodgkin Lymphoma [J]. PLoS One，2015，10（9）：e0138416.
[18] Wang J，Liu D，Liang X，et al. Construction of a recombinant eukaryotic human ZHX1 gene expression plasmid and the role of ZHX1 in hepatocellular carcinoma [J]. Mol Med Rep，2013，8（5）：1531-1536.
[19] Yamada K，Printz R，Osawa H，et al. Human ZHX1：cloning，chromosomal location，and interaction with transcription factor NF-Y [J]. Biochem Biophys Res Commun，1999，261（3）：614-621.
[20] Ogata-Kawata H，Yamada K，Uesaka-Yoshino M，et al. BS69，a corepressor interacting with ZHX1，is a bifunctional transcription factor [J]. Front Mol Biosci，2007，12：1911- 1926.
[21] Kim S，Park J，Choi M，et al. Zinc-fingers and homeoboxes 1 （ZHX1） binds DNA methyltransferase （DNMT） 3B to en- hance DNMT3B-mediated transcriptional repression [J]. Biochem Biophys Res Commun，2007，355（2）：318-323.
[22] Ma X，Huang M，Wang Z，et al. ZHX1 Inhibits Gastric Cancer Cell Growth through Inducing Cell-Cycle Arrest and Apoptosis [J]. J CANCER，2016，7（1）：60-68.
[23] Firlej V，Ladam F，Brysbaert G，et al. Reduced tumorigenesis in mouse mammary cancer cells following inhibition of Pea3-or Erm-dependent transcription [J]. J Cell sci，2008， 121：3393-402.
[24] Chen S，Yu X，Lei Q，et al. The SUMOylation of zinc- fingers and homeoboxes 1 （ZHX1） by Ubc9 regulates its stability and transcriptional repression activity [J]. J Cell Biochem，2013，114（10）：2323-2333.
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