Abstract：Objective To understand the genotypes of carbapenem-resistant Acinetobacter baumannii (CRAB) in patients with lower respiratory tract infection in the Department of Critical Care Medicine, Congjiang County People’s Hospital, Guizhou Province, and perform homology analysis on some strains. Methods A retrospective analysis of the proportion of CRAB in patients with lower respiratory tract infection in the Department of Intensive Care Medicine, Congjiang County People’s Hospital, Guizhou Province from January 2018 to December 2021, with 40 isolated CRAB strains as the research objects. VITEK2-compact automatic bacterial identification drug sensitivity system was used for bacterial identification and drug sensitivity. PCR was used to detect seven common carbapenemase genes (SHV, TEM, IMP, VIM, ADC, OXA-23, OXA-58). Multisite sequence typing (MLST) was used to analyze the homology of 40 strains. Results From 2018 to 2021, the detection rate of CRAB in patients with lower respiratory tract infection in the Department of Intensive Care Medicine of the Congjiang County People’s Hospital increased year by year. The 40 CRAB strains were 97.50% sensitive to Colistin and 80.00% sensitive to Amikacin. The detection of drug resistance genes revealed that the carrying rates of TEM, ADC, and OXA-23 genes were 47.5%, 22.5%, and 22.5%, respectively, as well as the presence of expression of multiple genotypes. The results of MLST typing showed that there were five sequence types, among which ST195 was the main type, accounting for 62.5%, followed by ST208 type, accounting for 20.0%. Conclusion The detection of CRAB in patients with lower respiratory tract infection in the intensive care unit is increasing year by year, and the choice of antibiotics is limited. Carrying TEM, ADC and OXA-23 genes is the main reason for the resistance of Acinetobacter baumannii to carbapenems. MLST results shows that there are clones in the ICU ward.
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