过敏性哮喘患儿血清lncRNA MEG3表达与Th17/Treg平衡的关系

doi:10.20047/j.issn1673-7210.2023.20.24

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摘要目的探讨过敏性哮喘患儿外周血长链非编码核糖核酸母系表达的基因3（lncRNA MEG3）表达与辅助性T细胞（Th）17/调节性T细胞（Treg）失衡的关系。方法选择2018年1月至2022年1月西安交通大学第一附属医院（以下简称“我院”）收治的75例过敏性哮喘患儿为哮喘组，另选择我院儿童保健门诊的49名健康儿童为对照组。检测两组Th17细胞百分比、Treg细胞百分比、Th17/Treg比值，lncRNA MEG3表达、白细胞介素（IL）-17、IL-22、IL-10、转化生长因子-β（TGF-β）水平及第1秒用力呼气容积（FEV1），计算FEV1与用力肺活量比值（FEV1/FVC）、FEV1占预计值百分比（FEV1%pred）。分析lncRNA MEG3表达与Th17/Treg及其细胞因子、肺功能的相关性。结果哮喘组lncRNA MEG3表达、Treg细胞百分比低于对照组，Th17细胞百分比、Th17/Treg高于对照组（P＜0.05）。哮喘组IL-17、IL-22水平高于对照组，TGF-β、IL-10水平低于对照组（P＜0.05）。哮喘组FEV1、FEV1/FVC、FEV1%pred低于对照组（P＜0.05）。哮喘组患儿lncRNA MEG3表达与Treg细胞百分比、IL-10、TGF-β、FEV1、FEV1/FVC、FEV1%pred呈正相关（r=0.502、0.415、0.491、0.493、0.428、0.497，P＜0.05），与Th17细胞百分比、Th17/Treg、IL-17、IL-22呈负相关（r=-0.501、-0.727、-0.631、-0.452，P＜0.05）。结论过敏性哮喘患者外周血lncRNA MEG3表达降低，且与Th17/Treg失衡及肺功能低下有关。

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	王星1　　李瑞婷2　　马小苗3▲

关键词 ：过敏性哮喘, 长链非编码核糖核酸, 母系表达的基因3, 免疫, 辅助性T细胞17, 调节性T细胞

Abstract：Objective To investigate the relationship between maternal long chain non-coding ribonucleic acid maternally expressed gene 3 (lncRNA MEG3) expression and T helper cell (Th) 17/ regulatory T cell (Treg) imbalance in children with allergic asthma. Methods A total of 75 children with allergic asthma admitted to the First Affiliated Hospital of Xi’an Jiaotong University (hereinafter referred to as “our hospital”) from January 2018 to January 2022 were selected as asthma group, and 49 healthy children from the Children’s health clinic of our hospital were selected as control group. The percentage of Th17 cells, the percentage of Treg cells, Th17/Treg ratio, lncRNA MEG3 expression, interleukin (IL)-17, IL-22，IL-10，transforming growth factor-β (TGF-β) levels, and forced expiratory volume in one second (FEV1) were detected, the ratio of FEV1 to forced vital capacity (FEV1/FVC), and the percentage of FEV1 to predicted value (FEV1%pred) were calculated. The correlation between lncRNA MEG3 expression and Th17/Treg and its cytokines, and lung function were analyzed. Results The expression of lncRNA MEG3, the percentage of Treg cells in asthma group were lower than those in control group, while the percentage of Th17 cells, Th17/Treg were higher than those in control group (P＜0.05). The levels of IL-17 and IL-22 in asthma group were higher than those in control group, while the levels of TGF-β and IL-10 were lower than those in control group (P＜0.05). The FEV1, FEV1/FVC, and FEV1%pred in asthma group were lower than those of control group (P＜0.05). The expression of lncRNA MEG3 in children with asthma was positively correlated with the percentage of Treg cells, IL-10, TGF-β, FEV1, FEV1/FVC, and FEV1%pred (r=0.502, 0.415, 0.491, 0.493, 0.428, 0.497, P＜0.05), and the percentage of Th17 cells, Th17/Treg, IL-17, and IL-22 were negatively correlated (r=-0.501, -0.727, -0.631, -0.452, P＜0.05). Conclusion The expression of lncRNA MEG3 in peripheral blood of patients with allergic asthma is decreased, which is related to Th17/Treg imbalance and low lung function.

Key words： Allergic asthma；Long non-coding ribonucleic acid Maternally expressed gene 3 Immune T helper cell 17 Regulatory T cell

基金资助:陕西省卫生健康科研项目（2018F004）。

通讯作者: ▲通讯作者

引用本文:

王星1　　李瑞婷2　　马小苗3▲. 过敏性哮喘患儿血清lncRNA MEG3表达与Th17/Treg平衡的关系[J]. 中国医药导报, 2023, 20(20): 113-116.
WANG Xing1 LI Ruiting2 MA Xiaomiao3▲. Relationship between serum lncRNA MEG3 expression and Th17/Treg balance in children with allergic asthma. 中国医药导报, 2023, 20(20): 113-116.

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[1] Schoettler N，Rodríguez E，Weidinger S，et al. Advances in asthma and allergic disease genetics：Is bigger always better? [J]. J Allergy Clin Immunol，2019，144（6）：1495-1506.
[2] Akar-Ghibril N，Casale T，Custovic A，et al. Allergic Endotypes and Phenotypes of Asthma[J]. J Allergy Clin Immunol Pract，2020，8（2）：429-440.
[3] Li C，Sheng A，Jia X，et al. Th17/Treg dysregulation in allergic asthmatic children is associated with elevated notch expression [J]. J Asthma，2018，55（1）：1-7.
[4] Ma B，Athari SS，Mehrabi Nasab E，et al. PI3K/AKT/mTOR and TLR4/MyD88/NF-κB Signaling Inhibitors Attenuate Pathological Mechanisms of Allergic Asthma [J]. Inflammation，2021，44（5）：1895-1907.
[5] Zhou F，Wang X，Wang L，et al. Genetics，Epigenetics，Cellular Immunology，and Gut Microbiota： Emerging Links With Graves’Disease [J]. Front Cell Dev Biol，2022，9：794912.
[6] 鲍一笑，陈爱欢，符州，等.儿童支气管哮喘诊断与防治指南(2016年版)[J].中华儿科杂志，2016，54（3）：167-181.
[7] 段庆宁，赵德育，严敏，等.5~18岁过敏性哮喘患儿螨皮下免疫治疗效应预测模型[J].中华儿科杂志，2022，60（4）：291-296.
[8] Zhang W，Lin C，Sampath V，et al. Impact of allergen immunotherapy in allergic asthma [J]. Immunotherapy，2018， 10（7）：579-593.
[9] Testa D，DI Bari M，Nunziata M，et al. Allergic rhinitis and asthma assessment of risk factors in pediatric patients：A systematic review [J]. Int J Pediatr Otorhinolaryngol，2020， 129：109759.
[10] 范爱红，米艳茹，代育中，等.过敏性哮喘患儿外周血Th17/Treg比值变化及免疫球蛋白、血IDO、白介素的相关性分析[J].临床肺科杂志，2021，26（7）：1041-1046.
[11] Bridges MC，Daulagala AC，Kourtidis A. LNCcation：lncRNA localization and function [J]. J Cell Biol，2021，220（2）：e202009045.
[12] Robinson EK，Covarrubias S，Carpenter S. The how and why of lncRNA function： An innate immune perspective [J]. Biochim Biophys Acta Gene Regul Mech，2020，1863（4）：194419.
[13] Ma L，Zhang Q，Hao J，et al. LncRNA PVT1 exacerbates the inflammation and cell-barrier injury during asthma by regulating miR-149 [J]. J Biochem Mol Toxicol，2020，34（11）： e22563.
[14] Ye S，Zhu S，Feng L. LncRNA ANRIL/miR-125a axis exhibits potential as a biomarker for disease exacerbation，severity，and inflammation in bronchial asthma [J]. J Clin Lab Anal，2020，34（3）：e23092.
[15] Al-Rugeebah A，Alanazi M，Parine NR. MEG3：an Oncogenic Long Non-coding RNA in Different Cancers [J]. Pathol Oncol Res，2019，25（3）：859-874.
[16] Li G，Liu Y，Meng F，et al. LncRNA MEG3 inhibits rheumatoid arthritis through miR-141 and inactivation of Akt/ mTOR signalling pathway [J]. J Cell Mol Med，2019，23（10）：7116-7120.
[17] Tang ZL，Zhang K，Lv SC，et al. LncRNA MEG3 suppresses PI3K/Akt/mTOR signalling pathway to enhance autophagy and inhibit inflammation in TNF-α-treated keratinocytes and psoriatic mice [J]. Cytokine，2021，148：155657.
[18] 李丽英，李海燕，陈海燕.支气管哮喘病儿外周血单核细胞中LncRNA MEG3的表达及其与肺功能、免疫功能的相关性研究[J].安徽医药，2021，25（4）：755-759.
[19] Feng Y，Yang C，Yan W. Expression of lncRNA MEG3 in asthma with different phenotypes and its relationship with course of disease [J]. Exp Ther Med，2020，19（3）：2211- 2217.
[20] Deng G，Song X，Fujimoto S，et al. Foxp3 Post-translational Modifications and Treg Suppressive Activity [J]. Front Immunol，2019，10：2486.
[21] Kumar R，Theiss AL，Venuprasad K. RORγt protein modifications and IL-17-mediated inflammation [J]. Trends Immunol，2021，42（11）：1037-1050.
[22] Baumjohann D. Diverse functions of miR-17-92 cluster microRNAs in T helper cells [J]. Cancer Lett，2018，423：147-152.
[23] Qiu YY，Wu Y，Lin MJ，et al. LncRNA-MEG3 functions as a competing endogenous RNA to regulate Treg/Th17 balance in patients with asthma by targeting microRNA-17/ RORγt [J]. Biomed Pharmacother，2019，111：386-394.