Abstract：Objective To establish UPLC fingerprint to compare the differences of chemical constituents in the main stem, lateral stem, and leaf of Andrographis paniculata, so as to provide a basis for the quality evaluation of Andrographis paniculata. Methods CORTECS UPLC T3 column (100 mm×2.1 mm, 1.6 μm ) was used; gradient elution was performed with acetonitrile-0.1 % phosphoric acid solution as mobile phase. The UPLC fingerprints of 14 batches (S1-S14) of main stem, lateral stem, and leaf of Andrographis paniculata were studied by similarity evaluation, heat map cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. Results In the established UPLC fingerprint, 13 common peaks were identified in the main stem, lateral stem, and leaf of Andrographis paniculata, and identified five characteristic peaks of luteolin-7-O-β-D glucuronide, andrographolide, neoandrographolide, 14-deoxyandrographolide, and anhydroandrographolide; except for S3, S7, and S9, the similarity between the UPLC fingerprints of main stem, lateral stem, and leaf of Andrographis paniculata and the corresponding control fingerprints was greater than 0.900; the results of heat map cluster analysis and principal component analysis could distinguish the main stem, lateral stem, and leaves; the orthogonal partial least squares discriminant analysis screened out four different components with VIP value＞1, P＜0.05. Conclusion This study reflects the differences of chemical constituents in different parts of Andrographis paniculata as a whole, comprehensively and truly, and is of great significance to the quality control and evaluation of Andrographis paniculata.
方朝缵 何荣荣 甘力帆 王瑜婷 陈仕妍 邓桂海 汪凯东 孙冬梅. 基于UPLC指纹图谱的穿心莲不同部位化学成分差异研究[J]. 中国医药导报, 2023, 20(19): 24-29.
FANG Chaozuan HE Rongrong GAN Lifan WANG Yuting CHEN Shiyan DENG Guihai WANG Kaidong SUN Dongmei. Study on chemical constituents of different parts of Andrographis paniculata based on UPLC fingerprint. 中国医药导报, 2023, 20(19): 24-29.
京公网安备 11010502046598号