Screening of excellent strains derived from 7 β，15 α-dihydroxy-18β- glycyrrhetinic acid by microbial transformation
MA Yuan1 SUN Peng2 WANG Heng3 LI Zhuofan3 CHEN Gang4 SHI Minghui4
1.Key Laboratory of Uygur Medicines, Xinjiang Uygur Autonomous Region Institute of Materia Medica, Xinjiang Uygur Autonomous Region, Urumqi 830004, China;
2.Medical Device Testing Center, Xinjiang Institutes of Drug Control, Xinjiang Uygur Autonomous Region, Urumqi 830054, China;
3.Department of Traditional Chinese Medicine, College of Pharmacy, Shihezi University, Xinjiang Uygur Autonomous Region, Shihezi 832000, China; 4.Analysis and Testing Center, Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medica and National Medica, Xinjiang Uygur Autonomous Region, Urumqi 830002, China
Abstract：Objective To screen the excellent strains through microbial transformation, using 7β，15α-dihydroxy-18β- glycyrrhetinic acid as the target product. Methods Ten fungi (CGMCC 3.149, CGMCC 3.193, CGMCC 3.3402, CGMCC 3.739, CGMCC 3.2462, CGMCC 3.2886, CGMCC 3.3400, CGMCC 3.577, CGMCC 3.970, and Zw-4) were used for preliminary screening and re-screening experiments of transformed strains, the content of 7β，15α-dihydroxy-18β-glycyrrhetinic acid in the converted liquid was determined by high performance liquid chromatography. The conversion rate of 7β，15α-dihydroxy-18β-glycyrrhetinic acid was used as the evaluation index and the strains with high conversion rate and consistent results of preliminary screening and re-screening experiments were selected as the excellent strains. 7β，15α-dihydroxy- 18β-glycyrrhetinic acid showed a good linear relationship in the range of 2.56~81.79 μg/ml. The strain with the highest conversion rate of preliminary screening experiment was CGMCC 3.3400. The strain with the highest conversion rate of re-screening experiment was CGMCC 3.970. Conclusion The excellent strain is CGMCC 3.970.
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