SOX4在结直肠癌中的表达及对生物学行为的影响

doi:10.20047/j.issn1673-7210.2023.13.05

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摘要目的探讨性别决定区Y框蛋白4（SOX4）在结直肠癌组织中的表达，并研究SOX4对人结直肠癌SW480细胞增殖、迁移和侵袭能力的影响。方法收集2018年1月至2019年1月于河北北方学院附属第一医院手术切除并经过病理证实的结直肠癌组织标本40例，采用逆转录定量聚合酶链反应（RT-qPCR）和Western blot分析SOX4在组织中的mRNA表达和蛋白表达水平。培养人结直肠癌SW480细胞，采用慢病毒转染技术将用阴性对照质粒（siRNA-NC组）和干扰质粒（siRNA-SOX4转染组）转染SW480细胞，另设置blank组，通过CCK-8法、细胞划痕实验、Transwell实验分别检测细胞增殖、迁移和侵袭能力。结果与癌旁正常组织比较，结直肠癌组织中SOX4 mRNA表达水平和蛋白表达水平升高。blank组与siRNA-NC组SOX4 mRNA表达水平、SOX4蛋白表达水平、SW480细胞增殖能力、SW480细胞划痕愈合率、SW480细胞侵袭能力比较，差异无统计学意义（P＞0.05）。与siRNA-NC组比较，siRNA-SOX4转染组中SOX4 mRNA表达水平、SOX4蛋白表达水平、SW480细胞的划痕愈合率、SW480细胞增殖能力、SW480细胞侵袭能力降低（P＜0.05）。结论 SOX4在结直肠癌组织中表达上调，且下调SW480细胞中SOX4的表达可明显抑制结直肠癌细胞增殖、迁移和侵袭能力。

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	张纲1　　王国强1　　王伟1　　武雪亮2　　郑海平1　　刘楠3　　张玉国1

关键词 ：结直肠癌, 性别决定区Y框蛋白4, 增殖, 迁移, 侵袭

Abstract：Objective To investigate the expression of sex-determining region Y-box 4 (SOX4) in colorectal cancer tissues, and to study the effects of SOX4 on the proliferation, migration, and invasion of human colorectal cancer SW480 cells. Methods A total of 40 specimens with pathologically confirmed colorectal cancer removed during surgical treatment in the First Affiliated Hospital of Hebei North University from January 2018 to January 2019 were collected. The mRNA and protein expression levels of SOX4 in tissues were analyzed by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot. Human colorectal cancer SW480 cells were cultured and transfected with negative control plasmid (siRNA-NC group) and interference plasmid (siRNA-SOX4 transfection group) using lentivirus transfection technique, and blank group was set up. Cell proliferation, migration, and invasion were detected by CCK-8 assay, cell scratch assay, and Transwell assay. Results Compared with paracancer normal tissue, the mRNA and protein expression levels of SOX4 in colorectal cancer tissue were increased. There was no significant difference in the mRNA expression level of SOX4, the protein expression level of SOX4, the proliferation ability of SW480 cells, the scratch healing rate of SW480 cells, and the invasion ability of SW480 cells between blank group and siRNA-NC group (P＞0.05). Compared with siRNA-NC group, the mRNA expression level of SOX4, the protein expression level of SOX4, the proliferation ability of SW480 cells, the scratch healing rate of SW480 cells, and the invasion ability of SW480 cells were decreased in siRNA-SOX4 transfection group (P＜ 0.05). Conclusion The expression of SOX4 is up-regulated in colorectal cancer tissues, and the down-regulation of SOX4 expression in SW480 cells can significantly inhibit the proliferation, migration, and invasion of colorectal cancer cells.

Key words： Colorectal cancer Sex-determining region Y-box 4 Proliferate Transfer Invasion

基金资助:河北省卫生健康委员会医学科学研究课题计划项目（20211329）。

通讯作者: 武雪亮（1984.9-），男，博士，副主任医师，副教授，硕士生导师；研究方向：消化道肿瘤的诊治。

引用本文:

张纲1　　王国强1　　王伟1　　武雪亮2　　郑海平1　　刘楠3　　张玉国1. SOX4在结直肠癌中的表达及对生物学行为的影响[J]. 中国医药导报, 2023, 20(13): 23-28.
ZHANG Gang1　WANG Guoqiang1　WANG Wei1　WU Xueliang2　ZHENG Haiping1　LIU Nan3　ZHANG Yuguo1. Expression of SOX4 in colorectal cancer and its effect on biological behavior. 中国医药导报, 2023, 20(13): 23-28.

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