Analysis of chemical constituents of Dracocephalum rupestre Hance based on UHPLC-QE-HRMS/MS method
WANG Feng1 DONG Peizhi2 LIAN Yunlan2
1.School of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine , Shanxi Province, Jinzhong 030619, China;
2.Institute of Drug Inspection Technology, Shanxi Inspection and Testing Center, Shanxi Province, Taiyuan 030031, China
Abstract：Objective To study the chemical constituents of Dracocephalum rupestre Hance by the ultra-high performance liquid chromatography-quadrupole electric field orbitrap with high-resolution mass spectrometry (UPLC-QE-HRMS/MS). Methods UHPLC was performed on a Hypersil GOLD aQ (2.1 mm×100 mm,1.9 μm) column at 40℃. The mobile phase was 0.1% formic acid water-acetonitrile with gradient elution at a flow rate of 0.4 ml/min. QE-HRMS/MS was scanned simultaneously by electron spray ionization positive and negative ions; the data were processed by the unknown identification and analysis software and compared with the first-order mass spectrometry quasi-molecular ion peak of the compound reference substance in the library, the main fragment ions of the second-order mass spectrometry and the relevant literature. Results A total of 31 chemical constituents were identified, of which 14 compounds were identified for the first time from Dracocephalum rupestre Hance. Conclusion UPLC-QE-HRMS/MS method can quickly and accurately identify a variety of chemical components in Dracocephalum rupestre Hance, and provides reference for quality control and pharmacodynamic material basis study.
王枫1 董培智2 连云岚2. UHPLC-QE-HRMS/MS法分析鉴定岩青兰的化学成分[J]. 中国医药导报, 2023, 20(12): 25-29.
WANG Feng1 DONG Peizhi2 LIAN Yunlan2. Analysis of chemical constituents of Dracocephalum rupestre Hance based on UHPLC-QE-HRMS/MS method. 中国医药导报, 2023, 20(12): 25-29.
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