Abstract：Objective To investigate the regulatory mechanism of modified Yinchenhao Decoction on rats with bronchial asthma (“asthma”for short) damp-heat syndrome based on metabolomics. Methods A total of 65 SPF male SD rats, 4 weeks old, (180±10)g, were divided into blank group (10 rats), model group (10 rats), western medicine group(15 rats), and traditional Chinese medicine group(15 rats). The damp-heat syndrome model was established by intraperitoneal injection of Ovalbumin Hydroxide saline suspension for sensitization, Ovalbumin to stimulate asthma, high-fat feeding, Escherichia coli gavaged, and placing in a damp-heat environment. After successfully modeling, the traditional Chinese medicine group was given modified Yinchenhao Decoction, the western medicine group was given Dexamethasone, the blank group and the model group were given normal saline for four weeks. The general state of rats was observed, different metabolites were screened, and metabolic pathways were analyzed. Results Compared with model group, after medication, the mental, dietary, and bowel status of traditional Chinese medicine group was improved, the response was sensitive, breathing was gradually stable, and oral and nasal secretions were reduced; the levels of 11, 14, 17-eicosanotrienoic acid, docosahexaenoic acid, docosapentaenoic acid, α-linolenic acid, L-linoleic acid and L-tryptophan, and L-lysine were increased in the traditional Chinese medicine group, while the levels of histidine were decreased. In the metabolic pathway, it has the strongest correlation with linoleic acid metabolism, followed by α-linolenic acid metabolism, arachidonic acid metabolism, tryptophan metabolism, histidine metabolism, arginine, and proline metabolism. Conclusion Modified Yinchenhao Decoction has therapeutic effect on damp-heat syndrome, and the mechanism may be related to the regulation of fatty acid and amino acid metabolism.
贺一玮 桑勉 孙洮玉 徐荣谦. 基于代谢组学探讨茵陈蒿汤加减方对支气管哮喘湿热证型大鼠的调控机制[J]. 中国医药导报, 2023, 20(12): 9-13.
HE Yiwei SANG Mian SUN Taoyu XU Rongqian. Study on the regulatory mechanism of modified Yinchenhao Decoction on rats with bronchial asthma damp-heat syndrome based on metabolomics. 中国医药导报, 2023, 20(12): 9-13.
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