儿童急性淋巴细胞白血病中Wnt通路抑制因子-1基因甲基化研究

doi:10.20047/j.issn1673-7210.2023.11.27

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摘要目的探讨Wnt通路抑制因子-1（Wif-1）表达及其甲基化状态在儿童急性淋巴细胞白血病（ALL）中的意义。方法选取徐州医科大学附属医院2017年6月至2022年2月新确诊的43例ALL患儿为试验组，20名骨髓移植供者为对照组。留取试验组、对照组骨髓血各3～5 ml，实时荧光定量聚合链反应法检测Wif-1 mRNA表达；Western blot法检测Wif-1蛋白表达；甲基化特异性聚合酶链反应法检测Wif-1基因甲基化状态。结果试验组Wif-1 mRNA及蛋白相对表达量低于对照组，差异有统计学意义（P＜0.05）；试验组Wif-1基因甲基化阳性率高于对照组，差异有统计学意义（P＜0.05）。结论儿童ALL存在Wif-1基因高甲基化。

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	韩伟王文鹏颜静李宣高吉照

关键词 ：急性淋巴细胞白血病, Wnt通路抑制因子-1, 甲基化, Wnt信号通路

Abstract：Objective To investigate the expression of Wnt inhibitory factor-1 (Wif-1) and its methylation status in childhood acute lymphoblastic leukemia (ALL). Methods A total of 43 newly diagnosed children with ALL from June 2017 to February 2022 at the Affiliated Hospital of Xuzhou Medical University were selected as the experimental group, 20 bone marrow transplant donors were selected as the control group. 3-5 ml of bone marrow blood from each of the experimental group and control group were collected, Wif-1 mRNA expression was detected by quantitative real-time fluorescence polymerase chain reaction; Wif-1 protein expression was detected by Western blot; Wif-1 gene methylation status was detected by methylation specific polymerase chain reaction. Results The relative expression of Wif-1 mRNA and protein in the experimental group was lower than that in the control group, and the difference was statistically significant (P＜0.05); the positive rate of Wif-1 gene methylation in the experimental group was higher than that in the control group, the difference was statistically significant (P＜0.05). Conclusion Wif-1 gene hypermethylation exists in childhood ALL.

Key words： Acute lymphoblastic leukemia Wnt inhibitory factor-1 Methylation Wnt signaling pathway

引用本文:

韩伟王文鹏颜静李宣高吉照. 儿童急性淋巴细胞白血病中Wnt通路抑制因子-1基因甲基化研究[J]. 中国医药导报, 2023, 20(11): 119-122.
HAN Wei WANG Wenpeng YAN Jing LI Xuan GAO Jizhao. Methylation of Wnt inhibitory factor-1 gene in childhood acute lymphoblastic leukemia. 中国医药导报, 2023, 20(11): 119-122.

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