Effects of Mifepristone combined with Xiaoliu Prescription on hemorheology and GPR30-EGFR signaling pathway in uterine fibroid patients with syndrome of qi stagnation and blood stasis
LI Qin1 RUAN Chengjuan2 ZHANG Ruiwen2 ZHANG Yanni2 CHEN Dongqiong3▲
1.The Basic Medical College, Yunnan University of Chinese Medicine, Yunnan Province, Kunming 650500, China;
2.the First Clinical Medical College, Yunnan University of Chinese Medicine, Yunnan Province, Kunming 650500, China;
3.the First Affiliated Hospital of Yunnan University of Chinese Medicine, Yunnan Province, Kunming 650021, China
Abstract：Objective To investigate the effects of Mifepristone combined with Xiaoliu Prescription on clinical efficacy, hemorheology, and GPR30-EGFR signaling pathway in intermyowall uterine fibroids patients with syndrome of qi stagnation and blood stasis. Methods Eighty intermyowall uterine fibroids patients with syndrome of qi stagnation and blood stasis admitted to the First Affiliated Hospital of Yunnan University of Chinese Medicine from December 2021 to March 2022 were selected and divided into control group (40 cases) and experimental group (40 cases) according to random number table method. The control group was treated with Mifepristone, and the experimental group was treated with Mifepristone combined with Xiaoliu Prescription. The treatment course of both groups was three months (withdrawal of the drug during menstrual period). The clinical efficacy and uterine volume, fibroid area, hemorheology indexes before and after treatment were compared between the two groups. Real-time quantitative PCR and Western blot were used to detect the mRNA and protein expression levels of G-protein-coupled receptor 30 (GPR30) and epidermal growth factor receptor (EGFR) in two groups. Results Four cases fell off in the experimental group. In the control group, five cases were shed and one case was eliminated. The clinical effect of experimental group was better than that of control group (P＜0.05). After treatment, uterine volume, fibroid area, and plasma viscosity in two groups were lower than those before treatment, and experimental group was lower than control group (P＜0.05). After treatment, the whole blood high and low shear viscosity values of experimental group were lower than those of experimental group before treatment and control group (P＜0.05). After treatment, the mRNA and protein expression levels of GPR30 and EGFR in experimental group were lower than those in control group (P＜0.05). There was no significant difference in the total incidence of adverse reactions between the two groups (P＞0.05). No abnormal changes of liver and kidney function were observed in both groups during treatment. Conclusion Mifepristone combined with Xiaoliu Prescription can effectively treat intermyowall uterine fibroids, reduce uterine volume and uterine fibroid area, improve hemorheology indexes, reduce GPR30 and EGFR expression, with high safety.
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