Protective effect of endothelium acupuncture preconditioning on neural function in rats with cerebral ischemia reperfusion
JIANG Wei1 LIU Huihui2▲ XIAO Xiaoyue3 CHU Junbo1 LIU Xu1 HONG Shenghan1
1.Teaching and Research Section of Massage, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150036, China;
2.Department of Acupuncture and Moxibustion, the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
3.College of Animal Medicine, the Northeast Agricultural University, Heilongjiang Province, Harbin 150006, China
Abstract：Abstract To study the protective effect of intracutaneous acupuncture preconditioning on neural function in rats with cerebral ischemia/reperfusion (CI/R). Method A total of 120 SD male rats aged eight weeks and weighing (230±10)g were divided into four groups (sham operation group, model group, electro-acupuncture group and endothelium acupuncture group), with 30 rats in each group. In the endothelium acupuncture group and electro-acupuncture group, the rats were pretreated with endothelium acupuncture and electro-acupuncture respectively, and then the middle cerebral artery occlusion (MCAO) reperfusion model of rats was prepared. The model group only accepts modeling treatment. In the sham operation group, only open neck surgery was performed, but no ligation was performed. Neurological impairment was analyzed by modified neurological deficit score (mNSS), and the levels of inflammatory cytokines interleukin(IL)-1β and IL-6 were measured by enzyme-linked immunosorbent assay. The expression levels of Caspase-3, Bax and BCL-2 were detected by Western blot. Results mNSS score in the model group was higher than that in the sham operation group (P＜0.05), mNSS scores in the electro-acupuncture group and endothelium acupuncture group were lower than that in the model group, and the differences were statistically significant (P＜0.05). The contents of IL-1β and IL-6 in model group were higher than those in sham operation group, and the differences were statistically significant (P＜0.05). The contents of IL-1β and IL-6 in electro-acupuncture group and endothelium acupuncture group were lower than those in model group, and the differences were statistically significant (P＜0.05). The protein levels of Caspase-3 and Bax in model group were higher than those in sham operation group, and the protein levels of BCL-2 were lower than those in sham operation group, and the differences were statistically significant (P＜0.05). The protein levels of Caspase-3 and Bax in electro-acupuncture group and endothelium acupuncture group were lower than those in model group, and the protein levels of BCL-2 were higher than those in model group, and the differences were statistically significant (P＜0.05). Conclusion Endothelium acupuncture pretreatment could significantly reduce the inflammatory factors of CI/R rats, inhibit neuronal apoptosis, and reduce the brain nerve function damage of CI/R rats. Moreover, intradermal needle pretreatment also has the advantages of convenience.
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