推拿对大鼠骨骼肌钝挫伤内质网应激和炎症的影响

doi:10.20047/j.issn1673-7210.2022.35.02

摘要
图/表
参考文献(25)
相关文章 (15)

全文: PDF (1192 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的探讨推拿对骨骼肌钝挫伤内质网应激和炎症的影响。方法 24只6周龄SPF级雄性SD大鼠，采用随机数字表法将其分为空白组、模型组、推拿非穴位组、推拿穴位组，每组6只。除空白组外，其余组建立大鼠腓肠肌钝挫伤模型。造模1 d后开始推拿干预，推拿穴位组点按阴陵泉，推拿非穴组点按非经非穴处，空白组和模型组不予任何干预。HE染色观察肌肉组织形态变化；酶联免疫吸附试验测定肿瘤坏死因子-α（TNF-α）、Ca2+-ATP酶（SERCA）含量；Western blot检测葡萄糖调节蛋白78（GRP78）及钙网蛋白（CRT）表达情况。结果与空白组比较，模型组肌纤维排列较为紊乱，可见明显断裂、溶解、瘢痕组织。与空白组比较，模型组TNF-α含量升高（P＜0.01）；与模型组比较，推拿穴位组、推拿非穴组TNF-α含量降低（P＜0.05）；与推拿非穴位组比较，推拿穴位组TNF-α含量低于推拿非穴位组（P＜0.05）。与空白组比较，模型组SERCA含量下降（P＜0.05）；与模型组比较，推拿穴位组、推拿非穴组SERCA含量升高（P＜0.05）。与空白组比较，模型组GRP78、CRT蛋白表达升高（P＜0.05）；与模型组比较，推拿穴位组、推拿非穴组GRP78、CRT蛋白表达降低（P＜0.05）；与推拿非穴位组比较，推拿穴组GRP78、CRT蛋白表达降低（P＜0.05）。结论推拿可有效改善大鼠骨骼肌钝挫伤并缓解内质网应激，其机制可能与上调SERCA及抑制内质网应激蛋白和炎症因子有关。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	林建平1 王浩2 刘海潮3 李明1 王诗忠1 陈少清3

关键词 ：推拿疗法, 骨骼肌损伤, 内质网应激, 炎症

Abstract：

[Abstract] Objective To explore the effect of massage on endoplasmic reticulum stress and inflammation in skeletal muscle contusion. Methods Twenty-four 6-week-old male SPF SD rats were divided into control group, model group, massage non-acupoint group, and acupoint massage group according to the random number table method, with six rats in each group. Except the blank group, the other groups were established the models of blunt trauma of gastrocnemius. One day after modeling, massage intervention was started. The massage acupoint group was selected as yīnlíngquán, the massage non-acupoint group was selected as non-meridian and non-acupoint, and the blank group and the model group did not receive any intervention. HE staining was used to observe the morphological changes of muscle tissue. The contents of tumor necrosis factor-α (TNF-α) and Ca2+-ATPase (SERCA) were determined by enzyme-linked immunosorbent assay. The expressions of glucose-regulating protein 78 (GRP78) and calcium-reticulin (CRT) were detected by Western blot. Results Compared with blank group, the arrangement of muscle fibers in model group was more disordered, with obvious fracture, dissolution and scar tissue. Compared with blank group, the content of TNF-α in model group was increased (P＜0.01). Compared with model group, TNF-α content in massage acupoint group and massage non-acupoint group was decreased (P＜0.05); compared with massage non-point group, the TNF-α content of massage acupoint group was lower than that of massage non-point group (P＜0.05). Compared with blank group, SERCA content in model group was decreased (P＜0.05). Compared with model group, SERCA content in massage acupoint group and massage non-acupoint group was increased (P＜0.05). Compared with blank group, the protein expressions of GRP78 and CRT in model group were increased (P＜0.05). Compared with model group, the protein expressions of GRP78 and cRT in massage acupoint group and massage non-acupoint group were decreased (P＜0.05); compared with the massage non-acupoint group, the protein expressions of GRP78 and CRT in the massage acupoint group were lower (P＜0.05). Conclusion Massage can improve skeletal muscle blunt contusion and relieve endoplasmic reticulum stress. The mechanism may be related to up-regulation of SERCA and inhibition of endoplasmic reticulum stress proteins and inflammatory factors.

Key words： Massage therapy Skeletal muscle injury Endoplasmic reticulum stress Inflammation

基金资助:国家自然科学基金资助项目（81904317、819739 24、81874501）；
福建省自然科学基金资助项目（2019J01360、2021J01817）。

通讯作者: 陈少清（1983-），男，医学博士，副教授；研究方向：脊柱及骨关节病康复研究。

作者简介: 林建平（1985-），女，医学博士；研究方向：脊柱及骨关节病康复研究。

引用本文:

林建平1 王浩2 刘海潮3 李明1 王诗忠1 陈少清3. 推拿对大鼠骨骼肌钝挫伤内质网应激和炎症的影响[J]. 中国医药导报, 2022, 19(35): 8-11,22.
LIN Jianping1 WANG Hao2 LIU Haichao3 LI Ming1 WANG Shizhong1 CHEN Shaoqing3. Effects of massage on endoplasmic reticulum stress and inflammation in skeletal muscle blunt contusion in rats. 中国医药导报, 2022, 19(35): 8-11,22.

链接本文:

https://www.yiyaodaobao.com.cn/CN/10.20047/j.issn1673-7210.2022.35.02 或 https://www.yiyaodaobao.com.cn/CN/Y2022/V19/I35/8

[1] Ish?覬i L，Krommes K，Husted RS，et al. Diagnosis，prevention and treatment of common lower extremity muscle injuries in sport-grading the evidence：a statement paper commissioned by the Danish Society of Sports Physical Therapy （DSSF）[J]. Br J Sports Med，2020，54（9）：528-537.
[2] Tomazoni SS，Frigo L，Dos Reis Ferreira TC，et al. Effects of photobiomodulation therapy and topical non-steroidal anti-inflammatory drug on skeletal muscle injury induced by contusion in rats-part 2：biochemical aspects [J]. Lasers Med Sci，2017，32（8）：1879-1887.
[3] Bohnert KR，McMillan JD，Kumar A. Emerging roles of ER stress and unfolded protein response pathways in skeletal muscle health and disease [J]. J Cell Physiol，2018，233（1）：67-78.
[4] Rossin F，Avitabile E，Catarinella G，et al. Reticulon-1C Involvement in Muscle Regeneration [J]. Metabolites，2021，11（12）：855.
[5] Afroze D，Kumar A. ER stress in skeletal muscle remodeling and myopathies [J]. FEBS J，2019，286（2）：379-398.
[6] Gallot YS，Bohnert KR. Confounding Roles of ER Stress and the Unfolded Protein Response in Skeletal Muscle Atrophy [J]. Int J Mol Sci，2021，22（5）：2567.
[7] 王镜淇，龚国清.内质网应激与炎症反应的研究进展[J].药学研究，2017，36（5）：279-282.
[8] Cardozo AK，Ortis F，Storling J，et al. Cytokines downregulate the sarcoendoplasmic reticulum pump Ca2+ ATPase 2b and deplete endoplasmic reticulum Ca2+，leading to induction of endoplasmic reticulum stress in pancreatic beta-cells [J]. Diabetes，2005，54（2）：452-461.
[9] 谢娇，邓多喜，陈英，等.推拿手法对骨骼肌损伤干预研究的进展[J].湖南中医杂志，2018，34（4）：199-201.
[10] Crisco JJ，Jokl P，Heinen GT，et al. A muscle contusion injury model-biomechanics，physiology，and histology [J]. Am J Sports Med，1994，22：702-710.
[11] Sakuma Y，Miyagi M，Inoue G，et al. Muscle injury in rats induces upregulation of inflammatory cytokines in injured muscle and calcitonin gene-related peptide in dorsal root ganglia innervating the injured muscle [J]. Muscle Nerve，2016，54（4）：776-782.
[12] 刘杏，魏晓菡，邓洁，等.骨骼肌钝挫伤兔模型制备与打击力度的关系[J].中国组织工程研究，2021，25（2）：5.
[13] 孙晶，王巍，田野，等.内质网应激相关活性氧及其机制[J].中国医药导报，2018，15（16）：42-44，54.
[14] Amici DR，Pinal-Fernandez I，Mázala DA，et al. Calcium dysregulation，functional calpainopathy，and endoplasmic reticulum stress in sporadic inclusion body myositis [J]. Acta Neuropathol Commun，2017，5（1）：24.
[15] Hu Q，Mao Y，Liu M，et al. The active nuclear form of SREBP1 amplifies ER stress and autophagy via regulation of PERK [J]. FEBS J，2020，287（11）：2348-2366.
[16] Periasamy M，Maurya SK，Sahoo SK，et al. Role of SERCA Pump in Muscle Thermogenesis and Metabolism [J]. Compr Physiol，2017，7（3）：879-890.
[17] Souza Jd，Gottfried C. Muscle injury：review of experimental models [J]. J Electromyogr Kinesiol，2013，23（6）：1253- 1260.
[18] 高天姣，马德慧，韩怡然，等.基于SIRT1/PGC-1α通路探讨腹部推拿对肥胖2型糖尿病模型大鼠抗炎作用机制研究[J].时珍国医国药，2021，32（8）：2028-2030.
[19] 黄健，闫兆东，周利民，等.仙花三香膏外敷联合推拿治疗风寒湿痹型膝骨关节炎临床疗效及对患者血清Dickkopf相关蛋白1和炎症因子的影响[J].河北中医，2021，43（3）：487-491.
[20] Zhang HY，Wang ZG，Lu XH，et al. Endoplasmic reticulum stress：relevance and therapeutics in central nervous system diseases [J]. Mol Neurobiol，2015，51（3）：1343-1352.
[21] Cao X，Lu XM，Tuo X，et al. Angiotensin-converting enzyme 2 regulates endoplasmic reticulum stress and mitochondrial function to preserve skeletal muscle lipid metabolism [J]. Lipids Health Dis，2019，18（1）：207.
[22] Walter F，O’Brien A，Concannon CG，et al. ER stress signaling has an activating transcription factor 6α （ATF6）- dependent “off-switch” [J]. J Biol Chem，2018，293（47）：18270-18284.
[23] Ibrahim IM，Abdelmalek DH，Elfiky AA. GRP78：A cell’s response to stress [J]. Life Sci，2019，226：156-163.
[24] Meyer BA，Doroudgar S. ER Stress-Induced Secretion of Proteins and Their Extracellular Functions in the Heart [J]. Cells，2020，9（9）：2066.
[25] Zhang G，Wang X，Bi X，et al. GRP78 （Glucose-Regulated Protein of 78 kDa） Promotes Cardiomyocyte Growth Through Activation of GATA4 （GATA-Binding Protein 4） [J]. Hypertension，2019，73（2）：390-398.