Abstract：Objective To analyze the drug resistance genotyping and drug susceptibility of methicillin-resistant staphylococcus aureus (MRSA) isolated from clinical practice. Methods A total of 254 strains of staphylococcus aureus (SA) isolated from various clinical specimens of inpatients and outpatients in Nanjing Gaochun People’s Hospital from March 2018 to July 2020 were selected. The mecA gene was detected by ordinary polymerase chain reaction (PCR). The MRSA isolates were genotyped using multiplex PCR technology for the staphylococcal chromosome mec box (SCCmec).Antimicrobial susceptibility test of SCCmec type Ⅱ and SCCmec type Ⅲ by kirby-Bauer (K-B) method. Results The results of Cefoxitin K-B showed that a total of 156 MRSA positive strains were detected in 254 SA strains, and the MRSA positive rate was 61.42%. The PCR amplification result of mecA gene showed that a total of 150 MRSA confirmed positive strains were detected out of 254 strains, and the detection rate was 59.06%. The results of multiplex PCR showed that among the 150 MRSA strains, 39 were SCCmec type Ⅱ, 86 were SCCmec type Ⅲ, two were SCCmec Ⅳa, two were SCCmec Ⅳb, and 21 were untyped. The resistance of SCCmec Ⅱ and SCCmec Ⅲ to Oxacillin, Ampicillin/Sulbactam, Cefoxitin, Meropenem, Penicillin G, and Cefazolin was 100.00%. SCCmecⅢ was resistant to Ciprofloxacin. The resistance of Erythromycin, Clindamycin, Chloramphenicol, Gentamicin, Tetracycline, and Rifampicin was higher than that of SCCmecⅡ (P＜0.05). Conclusion Using multiplex PCR technology to detect 150 strains of clinically isolated MRSA are mainly SCCmec type Ⅲ, followed by SCCmec type Ⅱ, and the two SCCmec genotypes have a diverse spectrum of drug-resistant bacteria.
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