Simultaneous determination of 7 components in Tenghuang Jiangu Pills by high performance liquid chromatography - quantitative analysis of multi-components by single-marker
ZHAO Yuwei1 NAN Minlun2 SI Yingqi3 LING Yuqi4 YIN Han4 HE Yufang5
1.Preparation Center, Affiliated Hospital of Changchun University of Chinese Medicine, Jilin Province, Changchun 130117, China;
2.Institute of Phytochemistry, Jilin Academy of Chinese Medicine Sciences, Jilin Province, Changchun 130012, China;
3.Jilin Province AOPU Jinzuan Technology Testing Service Co. , Ltd., Jilin Province, Changchun 130507, China;
4.College of Chinese Medicinal Materials, Jilin Agricultural University, Jilin Province, Changchun 130118, China;
5.School of Health Management, Changchun University of Chinese Medicine, Jilin Province, Changchun 130117, China
Abstract：Objective To establish a method of high performance liquid chromatography-quantitative analysis of multi-components by single-marker to simultaneously determine the contents of echinacoside, acteoside, naringin, epimedin A, epimedin B, epimedin C, icariin in Tenghuang Jiangu Pills. Methods The analysis of methanol extract of this drug was performed on a 25℃ thermostatic Agilent ZORBAX Extend C18(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of water (containing 0.1% phosphoric acid)-acetonitrile flowing at 1.0 ml/min in a gradient elution manner, and the detection wavelength was set at 280 nm．Using icariin as the internal reference substance, the relative correction factor of the other six components was established and the contents of each component were calculated．In order to validate the accuracy, the seven components in Tenghuang Jiangu Pills were determined and compared by both high performance liquid chromatography-quantitative analysis of multi-components by single-marker and an external standard method. Results The contents of seven components in 11 batches of Tenghuang Jiangu Pills were determined, seven constituents showed good linear relationships within their own ranges（r ≥ 0.999 7）, whose average recoveries were 97.70%-99.25% with the RSD of 0.53%-1.57%. The two methods did not show significant difference in assay results. Conclusion The method can be used for the simultaneous determination of seven components in Tenghuang Jiangu Pills, and the purpose of multi-index quality control was achieved．
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