Analysis of serum metabolomics of premature infants in different growth patterns
CHEN Ying1 WANG Yumei2 XU Wenying1 ZHANG Peiying1
1.Department of Child Health Care, Huai’an Maternal and Child Health Hospital Affiliated to Yangzhou University Medical College, Jiangsu Province, Huai’an 223002, China;
2.Department of Neonatal Disease Screening, Huai’an Maternal and Child Health Hospital Affiliated to Yangzhou University Medical College, Jiangsu Province, Huai’an 223002, China
Abstract：Objective To analyze the characteristics of serum metabolites and to search for differential metabolites and metabolic pathways of preterm infants in different growth patterns using metabolomics techniques. Methods Serum samples were collected from 55 preterm infants by six months of adjusted age who were hospitalized in Neonatal Intensive Care Unit and were followed by Department of Child Health Care of Huai’an Maternal and Child Health Hospital Affiliated to Yangzhou University from March 2019 to December 2020. They were divided into catch-up growth group (35 cases) and without catch-up growth group (20 cases) according to whether the Z-score difference between corrected age of six months and birth weight was ≥0.67. The serum metabolic profiles were detected by liquid chromatography-tandem mass spectrometry, the metabolites and metabolic pathways with significant differences between two groups were identified, respectively. Results A total of 28 serum differential metabolites were obtained. Among them, there were 27 upregulated substances, mainly amino acids, glycerol phospholipids, and organic heterocyclic compounds, etc.; down-regulated substance 1, belonging to fatty acids. The metabolite difference pathways mainly concentrated in metabolism and synthesis of amino acids. The most significant enrichment pathways were arginine and proline metabolism and glutathione metabolism. Conclusion Premature infants with catch-up growth in the early stage are more prone to lack of various nutrients, especially amino acids, because of their rapid growth. It should pay attention to the reasonable supplement of amino acids for preterm infants. Metabonomics analysis can provide scientific basis for nutritional evaluation and intervention of preterm infants.
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