Effect of moxibustion of zúsānlǐ and tàixī on cell apoptosis and expression of related proteins in decidua tissues of pregnant rats with kidney deficiency-corpus luteum inhibition of abortion model
HU Shan CHEN Pengdian▲ NING Yan▲ MA Fei LIU Yulei QIU Tingting QIN Xiaoling TANG Meng#br#
Department of Traditional Chinese Medicine, Shenzhen Maternity & Child Healthcare Hospital Affiliated to Southern Medical University, Guangdong Province, Shenzhen 518028, China
Abstract：Objective To observe the effect of moxibustion of zúsānlǐ and tàixī on cell apoptosis and expression of related proteins in decidua tissues of pregnant rats with kidney deficiency-corpus luteum inhibition of abortion model. Methods Thirty-two pregnant female SD rats (SPF, 8-10 weeks, 220-250 g) were divided into normal group, model group, Dydrogesterone group, and moxibustion group according to the random number table method, with eight rats in each group. The normal group was given normal saline intragastric administration, and the other groups were given “Hydroxyurea + Mifepristone” to prepare the kidney deficiency-corpus luteum inhibition of abortion model of pregnant SD rats. At the same time, the Dydrogesterone group was given Dydrogesterone Tablets 3.02 mg/kg, 2 ml/day by gavage. In the moxibustion group, bilateral zúsānlǐ and tàixī points were moxibusted for 15 min/(time·point) every day, and the samples were collected after nine days of continuous intervention. The number of embryos in each group was calculated and the diameter of uterus was measured. The cell apoptosis of decidua tissues of each group was detected by TUNEL method, and the apoptosis index (AI) value was calculated. The protein expressions of Bax, Bcl-2, and Caspase-3 in decidua tissues of each group were determined by immunohistochemistry. Results Compared with the normal group, the uterine diameter of the model group was decreased, and the difference was statistically significant (P ＜ 0.05). Compared with the model group, the uterine diameter of the moxibustion group was increased, and the difference was statistically significant (P ＜ 0.05). There were no significant differences in the total number of intrauterine embryos between normal group and model group, model group and moxibustion group (P ＞ 0.05). Compared with the normal group, the average OD value of Bax and Caspase-3 protein in the model group was increased, and the differences were statistically significant (P ＜ 0.05). Compared with the model group, the average OD value of Bax and Caspase-3 protein in the moxibustion group was decreased, and the differences were statistically significant (P ＜ 0.05). Compared with the normal group, the average OD value of Bcl-2 protein in the model group was decreased, and the difference was statistically significant (P ＜ 0.05). Compared with the model group, the average OD value of Bcl-2 protein in the moxibustion group was increased, and the difference was statistically significant (P ＜ 0.05). Compared with the normal group, the cell AI value of the model group was increased, and the difference was statistically significant (P ＜ 0.05). Compared with the model group, the cell AI value of the moxibustion group was decreased, and the difference was statistically significant (P ＜ 0.05). Conclusion Moxibustion at zúsānlǐ and tàixī may regulate cell apoptosis at the maternal-fetal interface by up-regulating the expression of Bcl-2 protein, down-regulating the overexpression of Bax and Caspase-3 protein and reducing the AI in decidua tissues of pregnant rats with kidney deficiency and corpus luteum inhibition of abortion
胡珊 陈鹏典▲ 宁艳▲ 马飞 刘昱磊 邱婷婷 覃晓玲 唐梦. 艾灸足三里、太溪对肾虚-黄体抑制流产模型孕鼠蜕膜组织细胞凋亡及相关蛋白表达的影响[J]. 中国医药导报, 2022, 19(33): 13-17.
HU Shan CHEN Pengdian▲ NING Yan▲ MA Fei LIU Yulei QIU Tingting QIN Xiaoling TANG Meng. Effect of moxibustion of zúsānlǐ and tàixī on cell apoptosis and expression of related proteins in decidua tissues of pregnant rats with kidney deficiency-corpus luteum inhibition of abortion model. 中国医药导报, 2022, 19(33): 13-17.
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