Experimental study on silymarin regulating proliferation and apoptosis of non-small cell lung cancer cells through Slit2
TANG Yin1 SONG Aiying1 HAN Xiao1 GENG Zhixin2▲#br#
1.Department of Oncology, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China; 2.Department of Rehabilitation, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China
Abstract：Objective To study the effect and mechanism of silymarin (SM) on proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells through Slit2. Methods NSCLC cell line A549 was cultured and divided into control group and SM groups with different doses (10, 20 and 40 mg/L), si-negative control (NC) group transfected with NC siRNA, si-NC+40 mg/L SM group transfected with NC siRNA and intervened with 40 mg/L SM, si-Slit2+40 mg/L SM group transfected with Slit2 siRNA. After 48 hours of intervention, cell proliferation A490 level, apoptosis rate, and the expression levels of Slit2, ROBO1, and C-X-C motif chemokine receptor 4 (CXCR4) were detected. Results The A490 level and apoptosis rate of A549 cells in 10, 20 and 40 mg/L SM groups were lower than those in control group, and the differences were statistically significant (P < 0.05). The A490 level of A549 cells in 20 mg/L SM group was lower than that in 10 mg/L SM group, and the apoptosis rate was higher than that in 10 mg/L SM group, the differences were statistically significant (P ＜ 0.05). The A490 level of A549 cells in 40 mg/L SM group was lower than that in 10 and 20 mg/L SM groups, and the apoptosis rate was higher than that in 10 and 20 mg/L SM groups, and the differences were statistically significant (P ＜ 0.05). Compared with the control group, the expression levels of Slit2 and ROBO1 in A549 cells of 10, 20 and 40 mg/L SM groups were increased, and the expression level of CXCR4 was decreased, and the differences were statistically significant (P ＜ 0.05). Compared with 10 mg/L SM group, the expression level of CXCR4 in A549 cells in 20 mg/L SM group was decreased, and the difference was statistically significant (P < 0.05). The expression levels of Slit2 and ROBO1 in A549 cells in 40 mg/L SM group were increased, and the expression level of CXCR4 was decreased, the differences were statistically significant (P ＜ 0.05). Compared with 20 mg/L SM group, the expression levels of Slit2 and ROBO1 in A549 cells in 40 mg/L SM group were increased, and the expression level of CXCR4 was decreased, and the differences were statistically significant (P ＜ 0.05). Compared with SI-NC group, the expression level of Slit2 and apoptosis rate of A549 cells in SI-NC +40 mg/L SM group were increased, and the level of A490 was decreased, and the differences were statistically significant (P ＜ 0.05). Compared with SI-NC +40 mg/L SM group, the expression level of Slit2 and apoptosis rate of A549 cells in SI-SLIT2 +40 mg/L SM group were decreased, and the level of A490 was increased, and the differences were statistically significant (P ＜ 0.05). Compared with si-NC group, the expression level of ROBO1 in A549 cells of si-NC+40 mg/L SM group was increased, and the expression level of CXCR4 was decreased, and the differences were statistically significant (P ＜ 0.05). Compared with si-NC+40 mg/L SM group, the expression level of ROBO1 in A549 cells in si-slit2 +40 mg/L SM group was decreased, and the expression level of CXCR4 was increased, and the differences were statistically significant (P ＜ 0.05). Conclusion SM inhibits the proliferation and CXCR4 expression， promotes cell apoptosis in NSCLC cell by activating Slit2 / ROBO1 pathway.
唐寅1 宋爱英1 韩笑1 耿智馨2▲. 水飞蓟素通过Slit2调控非小细胞肺癌细胞增殖及凋亡的实验研究[J]. 中国医药导报, 2022, 19(28): 23-27,45.
TANG Yin1 SONG Aiying1 HAN Xiao1 GENG Zhixin2▲. Experimental study on silymarin regulating proliferation and apoptosis of non-small cell lung cancer cells through Slit2. 中国医药导报, 2022, 19(28): 23-27,45.
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