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基于p38 MARK信号通路探讨益气生肌合剂联合三乙醇胺乳膏对压疮大鼠模型皮损及血清炎症因子的影响
唐玉利1      刘欣1      廖若夷2
1.湖南中医药大学第一附属医院老年病科,湖南长沙   410208;
2.湖南中医药大学第一附属医院护理部,湖南长沙   410208
Discussion on the effect of Yiqi Shengji Mixture combined with Triethanolamine Cream on skin lesions and serum inflammatory factors in pressure ulcer rat model based on p38 MARK signaling pathway
TANG Yuli1   LIU Xin1   LIAO Ruoyi2
1.Department of Geriatrics, the First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan Province, Changsha   410208, China; 
2.Department of Nursing, the First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan Province, Changsha   410208, China
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摘要 目的 探讨益气生肌合剂联合三乙醇胺乳膏对压疮大鼠模型皮损、血清炎症因子的影响及作用机制。方法 50只SPF级SD大鼠(雌雄各半,6~7周龄,180~200 g)按照随机数字表法分为假手术组、模型组、三乙醇胺乳膏组、益气生肌组、联合用药组,每组10只。模型组、三乙醇胺乳膏组、益气生肌组、联合用药组模拟缺血再灌注法造模,假手术组仅同部位埋入磁铁不予加压。假手术组与模型组给予生理盐水0.4 ml灌胃;三乙醇胺乳膏组予三乙醇胺乳膏外用;益气生肌组予12.98 g/(kg·d)益气生肌合剂灌胃;联合用药组予三乙醇胺乳膏外用及益气生肌合剂灌胃,各组均连续给药8 d。记录各组创面情况,血清炎症因子水平及创面p38 MARK信号通路相关蛋白表达情况。 结果 三乙醇胺乳膏组、益气生肌组、联合用药组造模第8天皮损面积低于模型组,联合用药组造模第8天皮损面积低于三乙醇胺乳膏组、益气生肌组(P < 0.05)。模型组白细胞介素(IL)-2、IL-12、C反应蛋白(CRP)血清炎症因子水平高于假手术组(P < 0.05);三乙醇胺乳膏组、益气生肌组、联合用药组IL-2、IL-12、CRP血清炎症因子水平均低于模型组且联合用药组IL-2、IL-12、CRP血清炎症因子水平低于三乙醇胺乳膏组与益气生肌组(P < 0.05)。假手术组皮损组织p38丝裂素活化蛋白激酶(p38 MARK)、血管内皮生长因子(VEGF)、基质金属蛋白酶9(MMP-9)蛋白表达水平低于模型组(P < 0.05)。三乙醇胺乳膏组、益气生肌组、联合用药组皮损组织p38 MARK、VEGF、MMP-9蛋白表达水平均低于模型组,且联合用药组皮损组织p38 MARK、VEGF、MMP-9蛋白表达水平低于三乙醇胺乳膏组与益气生肌组(P < 0.05)。 结论 益气生肌合剂与三乙醇胺乳膏联用可有效修复压疮大鼠模型皮损,降低血清炎症因子水平。
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唐玉利1 刘欣1 廖若夷2
关键词 压疮益气生肌合剂p38 MARK信号通路炎性因子机制研究    
Abstract:Objective To investigate the effect and mechanism of Yiqi Shengji Mixture combined with Triethanolamine Cream on skin lesions and serum inflammatory factors in a rat model of pressure ulcer. Methods Fifty SPF SD rats (half male and half male, 6-7 weeks old, 180-200 g) were divided into sham operation group, model group, Triethanolamine Cream group, Yiqi Shengji group, and combined medication group by random number table method, with 10 rats in each group. The model group, Triethanolamine Cream group, Yiqi Shengji group, combined medication group were simulated by ischemia-reperfusion method. in the sham operation group, the magnet was only embedded in the same site without pressure. Sham operation group and model group were given normal saline 0.4 ml by gavage; Triethanolamine Cream group was given Triethanolamine Cream for external use; Yiqi Shengji group was given 12.98 g/(kg·d) Yiqi Shengji Mixture by gavage; combined medication group received Triethanolamine Cream for external use and Yiqi Shengji Mixture by gavage, all groups were given for eight days. Wound conditions, serum inflammatory factor levels, and wound P38 MARK signaling pathway related protein expression were recorded in each group. Results The skin lesion area of Triethanolamine Cream group, Yiqi Shengji group, and combined medication group on the 8th day of modeling was lower than that of model group, and the skin lesion area of combined medication group on the 8th day of modeling was lower than that of Triethanolamine Cream group and Yiqi Shengji group(P < 0.05). The serum levels of interleukin (IL)-2, IL-12, and C-reactive protein (CRP) in the model group were higher than those in the sham operation group (P < 0.05). The serum inflammatory factor levels of IL-2, IL-12, and CRP in Triethanolamine Cream group, Yiqi Shengji group and combined medication were lower than those in model group, and the serum inflammatory factor levels of IL-2, IL-12, and CRP in combined medication group were lower than those in Triethanolamine Cream group and Yiqi Shengji group (P < 0.05). The protein expressions of p38mitogen-activatedproteinkinase (p38 MARK), vascular endothelial growth factor (VEGF), and matrix metalloproteinases (MMP-9) in the skin lesions of the sham operation group were lower than those of the model group (P < 0.05). The protein expression levels of p38 MARK, VEGF, and MMP-9 in skin lesions in Triethanolamine Cream group, Yiqi Shengji group and combination drug group were lower than those in model group, and the protein expression levels of p38 MARK, VEGF, and MMP-9 in skin lesions in combination drug group were lower than those in Triethanolamine Cream group and Yiqi Shengji group (P < 0.05). Conclusion The combination of Yiqi Shengji Mixture and Triethanolamine Cream can effectively repair the skin lesions of the pressure ulcer rat model and reduce the level of serum inflammatory factors.
Key wordsPressure ulcer;Yiqi Shengji Mixture    p38 MARK signal pathway    Inflammatory factor    Mechanism study
    
基金资助:湖南省科技厅“湖南省临床医疗技术创新引导计划项目”(2020SK51404)。
通讯作者: 廖若夷(1970-),女,博士,主任护师;研究方向:护理管理,护理教学。   
引用本文:   
唐玉利1 刘欣1 廖若夷2. 基于p38 MARK信号通路探讨益气生肌合剂联合三乙醇胺乳膏对压疮大鼠模型皮损及血清炎症因子的影响[J]. 中国医药导报, 2022, 19(25): 24-27,37.
TANG Yuli1 LIU Xin1 LIAO Ruoyi2. Discussion on the effect of Yiqi Shengji Mixture combined with Triethanolamine Cream on skin lesions and serum inflammatory factors in pressure ulcer rat model based on p38 MARK signaling pathway. 中国医药导报, 2022, 19(25): 24-27,37.
链接本文:  
https://www.yiyaodaobao.com.cn/CN/10.20047/j.issn1673-7210.2022.25.05     或     https://www.yiyaodaobao.com.cn/CN/Y2022/V19/I25/24

 

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