Abstract：Objective To investigate the regulation of prostaglandin E receptor 2 (EP2) / Nod-like receptor protein 3 (NLRP3) and its anti-inflammatory mechanism in primary dysmenorrhea rats by sparrow-pecking moxibustion. Methods A total of 24 female SD rats were divided into blank group, model group, sprrow-pecking moxibustion group, and agonist group, with six rats in each group. Except blank group, the primary dysmenorrhea model was established for the other three groups, blank group received the same dose of normal saline daily. Blank group and model group were not treated; in sparrow-pecking moxibustion group, sānyīnjiāo and gu?觀nyuán acupoints were selected for treatment after daily modeling; agonist group was intraperitoneally injected with Treprostinil daily after modeling, followed by sparrow-pecking moxibustion treatment, which was the same as the sparrow-pecking moxibustion group. The latent time and number of torsion were observed in each group; HE staining was used to observe the pathological conditions of uterus; the expressions of EP2, NLRP3, and caspase-1 proteins were detected by immunohistochemistry. Results Compared with blank group, the latent time was shortened and the number of torsion was increased in model group (P < 0.01); compared with model group, the latent time was prolonged and the number of torsion was decreased in sparrow-pecking moxibustion group (P < 0.01); compared with sparrow-pecking moxibustion group, the latent time was shortened and the number of torsion was increased in agonist group (P < 0.01). In blank group, the uterine tissue morphology was regular, there were a few lymphocytes in the interstitium of muscle cells, and no neutrophil infiltration; in model group and agonist group, small uterine vessels showed dilation and hyperemia, and inflammatory cells such as lymphocytes and eosinophils were infiltrated outwards as focal groups; in sparrow-pecking moxibustion group, the degree of interstitial hyperemia and edema and infiltration of inflammatory cells were less than that in model group, a few inflammatory cells infiltrated between muscle walls and only a few small blood vessels hyperemia were observed. Compared with blank group, the protein expressions of EP2, NLRP3, and caspase-1 were increased in model group (P < 0.01); compared with model group, the protein expressions of EP2, NLRP3, and caspase-1 were decreased in sparrow-pecking group (P < 0.01); compared with sparrow-pecking group, the protein expressions of NLRP3 and caspase-1 were increased in agonist group, while the protein expression of EP2 in sparrow-pecking group and agonist group was not significantly different (P > 0.05). Conclusion Sparrow-pecking moxibustion can improve the infiltration of inflammatory cells in the uterus, and regulate the inflammatory response of dysmenorrhea rats by regulating EP2/NLRP3 signal pathway.
胡静 卫弯 高慧 徐刚 杨华元. 基于EP2/NLRP3信号通路探讨雀啄灸对原发性痛经大鼠的影响[J]. 中国医药导报, 2022, 19(22): 5-8,19.
HU Jing WEI Wan GAO Hui XU Gang YANG Huayuan. Effect of sparrow-pecking moxibustion on primary dysmenorrhea rats based on EP2/NLRP3 signal pathway. 中国医药导报, 2022, 19(22): 5-8,19.
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