Structure prediction and immune simulation of Brucella Omp2a-BtpB multi-epitope vaccine
LI Min1 ZHU Yuejie2 HU Jinwei1 GU Ting1 YU Mingkai3 CHEN Zhiqiang3 DING Jianbing3 ZHANG Fengbo1#br#
1.Medical Laboratory Center, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China; 2.Reproductive Pregnancy Center, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China; 3.Department of Immunology, Basic Medical College, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China
Abstract：Objective To predict and analyze T and B cell dominant epitopes of Omp2a and BtpB of Brucella by bioinformatics and construct a novel multi-epitope vaccine. Methods The amino acid sequences of the proteins, which were obtained from UniProt Database. Furthermore, the 3D structure of the proteins was builded by I-TASSER. Then being selected dominant epitopes and adjuvants to construct an multi-epitope vaccine and perform immune simulation. Results Two B cell dominant epitopes, five CTL cell dominant epitopes and three Th cell dominant epitopes were obtained in Omp2a. One B cell dominant epitope, five CTL cell dominant epitopes and one Th cell dominant epitope were obtained in BtpB. The constructed multi-epitope vaccine was a hydrophilic stable protein. Conclusion A novel Brucella multi-epitope vaccine is designed by using a bioinformatics approach, providing a theoretical basis for further research on Brucella vaccines.
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