Research on the action mechanism of Bushen Ankun Decotion in the treatment premature of ovarian insufficiency based on network pharmacology#br#
XIE Xiaohui1 LIU Jinxing2
1.The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Shandong Province, Jinan 250000, China;
2.Department of Gynecology, Shandong Provincial Hospital of Traditional Chinese Medicine, Shandong Province, Jinan 250000, China
Abstract：Objective To study the action mechanism of Bushen Ankun Decotion in the treatment of premature ovarian insufficiency (POI ) based on network pharmacology. Methods With the help of traditional Chinese medicine system pharmacology database and analysis platform (TCMSP ), the effective components and related targets were obtained; the standard names of the targets were found according to the Uniprot database; based on Cytoscape software, the network diagram of traditional Chinese medicine component-target was obtained. According to Genecards and OMIM, target genes of disease were obtained. Protein-protein interaction network of core target proteins was obtained by using STRING database and Cytoscape software. DAVID database and Cluego software were used for GO enrichment analysis and KEGG enrichment analysis. PubChem database, Uniprot database, AutoDock Tools software and DS ViewerPro software were used for molecular docking of main components and key targets. Results According to the screening conditions, 150 active components and 1088 targets of Bushen Ankun Decotion were obtained. 2718 POI target genes were retrieved, and 166 common targets of Bushen Ankun Decoction and disease were obtained. The results of molecular docking showed that the main compounds and their corresponding target proteins had good binding activities. GO functional enrichment analysis showed that it was mainly involved in cell surface. KEGG pathway enrichment analysis, a total of 228 pathways were enriched. Conclusion The mechanism of Bushen Ankun Decoction in the treatment of POI reflects the characteristics of multi-component, multi-target and multi-channel of traditional Chinese medicine, and provides a theoretical basis for further study of related diseases and drugs.
解小慧1 刘金星2. 基于网络药理学研究补肾安坤汤治疗早发性卵巢功能不全的作用机制[J]. 中国医药导报, 2022, 19(9): 31-36.
XIE Xiaohui1 LIU Jinxing2. Research on the action mechanism of Bushen Ankun Decotion in the treatment premature of ovarian insufficiency based on network pharmacology#br#. 中国医药导报, 2022, 19(9): 31-36.
[1] 陈士岭，周星宇.早发性卵巢功能不全的临床诊治进展[J].山东大学学报：医学版，2018，56（4）：1-7.
[2] Webber L，Davies M，Anderson R，et al. ESHRE Guideline： management of women with premature ovarian insufficiency [J]. Hum Reprod，2016，31（5）：926-937．
[3] 张雨，李恒，李克宁，等.复方中药网络药理学的研究进展[J].中成药，2018，40（7）：1584-1588．
[4] 陈海彬，周红光，李文婷，等.网络药理学——中药复方作用机制研究新视角[J].中华中医药杂志，2019，34（7）：2873-2876.
[5] Liu H，Wang J，Zhou W，et al. Systems approaches and polypharmacology for drug discovery from herbal medicines： An example using licorice [J]. J Ethnopharmacol，2013， 146（3）：773-793.
[6] 李冰涛，章辉，肖思雨，等.基于网络药理学的葛根治疗冠心病作用机制研究[J].中国中医药信息杂志，2019，26（10）：96-100.
[7] 应敏，王颖，刘金坤.蠲痹汤治疗类风湿关节炎的网络药理学分析[J].中国药业，2019，28（17）：1-7.
[8] 姚卫卫，刘金星.刘金星教授治疗卵巢早衰经验总结[J].世界最新医学信息文摘，2017，17（34）：146，149.
[9] 李炜，牛建昭，王继峰，等.槲皮素的植物雌激素作用及其受体机制研究[J].中国医院药学杂志，2015，35（2）：91-95.
[10] 庄小兰，傅玉才，许锦阶，等.槲皮素对大鼠卵巢发育和卵巢寿命的影响[J].中国组织工程研究与临床康复，2010， 14（15）：2727-2730.
[11] Jiao W，Xin Q，Qiang G，et al. Quercetin increases the antioxidant capacity of the ovary in menopausal rats and in ovarian granulosa cell culture in vitro [J]. J Ovarian Res，2018，11（1）：51.
[12] 唐晓静.党参炔苷和木犀草素对卵巢颗粒细胞激素分泌的影响及其作用机制[D].北京：中央民族大学，2015.
[13] 曾莉萍，徐贤柱，饶华，等.杜仲叶β-谷甾醇对成骨细胞和卵巢颗粒细胞的影响[J].时珍国医国药，2012，23（5）：1051-1053.
[14] Hong Y，Yin Y，Tan Y，et al. The Flavanone，Naringenin， Modifies Antioxidant and Steroidogenic Enzyme Activity in a Rat Model of Letrozole-Induced Polycystic Ovary Syndrome [J]. Med Sci Monit，2019，25：395-401.
[15] Yogo K，Ogawa T，Akiyama M，et al. PKA Implicated in the Phosphorylation of Cx43 Induced by Stimulation with FSH in Rat Granulosa Cells [J]. J Reprod Dev，2006，52（3）：321-328.
[16] Wang Z，Shi F. Phosphodiesterase 4 and compartmentalization of cyclic AMP signaling [J]. Chinese Sci Bull，2007（1）：34-46.
[17] 郑梦雪.促卵泡刺激素通过调控颗粒细胞分泌干细胞因子促进卵泡发育成熟的研究[D].银川：宁夏医科大学，2017.
[18] Hillier SG，Tetsuka M. 3 Role of androgens in follicle maturation and atresia [J]. Baillieres Clin Obstet Gynaecol，1997，11（2）：249-260.
[19] 张博，苏杭，任献青，等.基于高通量转录组测序的菟丝子黄酮改善雷公藤多苷片致大鼠生殖损伤的机制研究[J].中国中药杂志，2019，16：3478-3485.
[20] Detti L，Uhlmann RA，Fletcher NM，et al. Endometrial signaling pathways during ovarian stimulation for assisted reproduction technology [J]. Fertil Steril，2013，100（3）：889-894.
[21] Makrigiannakis A，Amin K，Coukos G，et al. Regulated expression and potential roles of p53 and Wilms’ tumor suppressor gene （WT1） during follicular development in the human ovary [J]. J Clin Endocrinol Metab，2000，85（1）：449-459.
[22] 何连利.Rg1延缓D-gal POF小鼠模型生殖系统病理损害、改善生殖功能及其机制的研究[D]. 重庆：重庆医科大学，2017.
[23] 张晨曦.MDM2-p53信号通路在卵泡的存活和发育过程中的作用研究[D].南京：南京大学，2016.
[24] 李德保，周莉，孙祖越.早发性卵巢功能不全相关信号通路研究进展[J].中国比较医学杂志，2020，30（2）：121-127.
[25] 陈薪.卵巢衰老诊疗新策略的探索性研究[D].广州：南方医科大学，2016.
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