溃疡性结肠炎中巨噬细胞差异基因及功能分析
陈梦雪 王宏刚 谢睿▲
江苏省淮安市第一人民医院消化内科,江苏淮安 223300
Analysis of differential genes and functions of macrophages in ulcerative colitis #br#
CHEN Mengxue WANG Honggang XIE Rui▲
Department of Gastroenterology, Huaian First People’s Hospital, Jiangsu Province, Huaian 223300, China
摘要 目的 研究溃疡性结肠炎(UC)肠道巨噬细胞的基因表达差异和生物学功能。 方法 选取2019年1月至12月前往the VA San Diego医疗机构就诊的7例UC患者和7名健康对照者为研究对象。分析直肠黏膜单细胞测序数据,比较巨噬细胞的差异表达基因,进行基因本体(GO)功能富集和京都基因和基因组数据库(KEGG)信号通路富集分析。 结果 过滤细胞,确定巨噬细胞群。筛选出UC组和对照组肠黏膜差异基因31个,包括TIMP1、IFITM3、IGHG1等。对差异基因进行GO和KEGG富集分析,发现主要富集于氧化应激生物学过程和IL-17信号通路。 结论 肠道巨噬细胞的氧化应激和IL-17信号通路可能参与UC疾病过程。
关键词 :
溃疡性结肠炎 ,
单细胞测序 ,
巨噬细胞 ,
氧化应激 ,
白细胞介素-17
Abstract :Objective To study the difference in gene expression and biological functions of intestinal macrophages in ulcerative colitis (UC). Methods Seven UC patients and seven healthy controls who visited the VA San Diego Healthcare facility from January to December 2019 were selected as the study subjects. The colonic mucosal single cell sequencing data were analyzed, and the differentially expressed genes in macrophages were compared, then gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were performed. Results The cells were filtered and the cluster of macrophage was determined. A total of 31 differentially expressed genes were selected in intestinal mucosa of UC group and control group, including TIMP1, IFITM3, IGHG1, etc. GO and KEGG enrichment analysis of differentially expressed genes showed mainly enriched in the biological process of oxidative stress and IL-17 signaling pathway. Conclusion The oxidative stress and IL-17 signaling pathway of intestinal macrophages may be involved in the process of UC disease.
Key words :
Ulcerative colitis
Single cell sequencing
Macrophages
Oxidative stress
Interleukin-17
基金资助: 江苏省卫生健康委员会科研课题(H2018082);
江苏省淮安市科学技术局市级科技计划专项基金项目(HAB201926)。
通讯作者:
▲通讯作者
作者简介 : 陈梦雪(1993.12-),女,硕士;研究方向:溃疡性结肠炎方面的诊治。
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