Abstract：Objective To study the expression of microRNA (miR)-340-5p, proto-oncogene c-Myc and transcription factor-4 (TCF-4) in laryngeal cancer tissues, and to analyze the correlation between the expression of them and the clinicopathological characteristics of laryngeal cancer. Methods A total of 102 patients with laryngeal cancer treated in Panjin Liaoyou Baoshihua Hospital from February 2015 to November 2020 were selected as the research subjects. The expressions of miR-340-5p, c-Myc, TCF-4 in cancer tissues and para-cancer tissue of 102 patients with laryngeal cancer were detected by real time fluorescence quantitative reverse transcription polymerase chain reaction. The correlation between the expressions of them and clinicopathological characteristics was statistically analyzed, and the correlation between the expressions of them was analyzed by Pearson linear correlation. Results Compared with para-cancer tissues, the relative expression levels of miR-340-5p in cancer tissues were significantly lower, while the relative expression levels of c-Myc and TCF-4 were significantly higher (P ＜ 0.05). There were statistically significant differences in the expressions of miR-340-5p, c-Myc, and TCF-4 in laryngeal cancer tissues with different tumor stages (P ＜ 0.05), while there were no statistically significant differences in gender, age, parting, tumor differentiation degree and whether or not lymph node metastasis (P ＜ 0.05). The expression of miR-340-5p was negatively correlated with c-Myc and TCF-4 (r = -0.549, -0.452, P < 0.05). Conclusion The expression of miR-340-5p is decreased in laryngeal cancer tissues, while the expression of c-Myc and TCF-4 is up-regulated. miR-340-5p, c-Myc, and TCF-4 are all related to the tumor stages of laryngeal cancer and are involved in the occurrence and development of laryngeal cancer.
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