苦金片对脂多糖诱导巨噬细胞炎症反应的影响

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摘要目的以脂多糖（LPS）刺激小鼠单核巨噬细胞建立炎症反应细胞模型，探讨苦金片对炎症反应的影响及可能机制。方法细胞分为对照组、LPS组、苦金片组，LPS组细胞予LPS（终浓度1 μg/ml）刺激12 h，苦金片组于LPS刺激后加苦金片（1 mg/ml）刺激4 h，对照组不予特殊处理。检测白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、转化生长因子-β（TGF-β）、自噬相关蛋白5（ATG5）、膜型微管相关蛋白1轻链3Ⅱ（LC3Ⅱ）mRNA及TNF-α、caspase-3、LC3Ⅱ、p62蛋白。结果与对照组比较，LPS组IL-6、TNF-α、TGF-β、ATG5、LC3Ⅱ mRNA表达水平显著增加，差异有统计学意义（P ＜ 0.05）；与对照组比较，LPS组TNF-α、LC3Ⅱ、p62和caspase-3蛋白表达水平增加，差异有统计学意义（P ＜ 0.05）。苦金片组IL-6、TNF-α、TGF-β、ATG5、LC3Ⅱ mRNA表达水平明显低于LPS组，差异有统计学意义（P ＜ 0.05）；苦金片组TNF-α、LC3Ⅱ、p62和Caspase-3蛋白表达水平明显低于LPS组，差异有统计学意义（P ＜ 0.05）。结论苦金片可显著减轻LPS诱导的细胞炎症反应，减少凋亡，提高细胞活力；细胞自噬参与了苦金片的抗炎过程。

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	刘晴朱春雪刘承雨王一成何美娟何嫣婕黄汉鹏▲

关键词 ：苦金片, 脂多糖, 巨噬细胞, 炎症, 细胞自噬

Abstract：Objective To investigate the effect of Kujin Tablets on the inflammatory response and its possible mechanism, the cell model of inflammatory response was established by lipopolysaccharide (LPS) stimulation of mononuclear macrophages in mice. Methods The cells were divided into control group, LPS group and Kujin Tablets group. The cells in LPS group were stimulated with LPS (final concentration of 1 μg/ml) for 12 h, and the Kujin Tablets group was stimulated with Kujin Tablets (1 mg/ml) for 4 h after LPS stimulation. The control group was not treated with special treatment. Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), autophagy associated protein 5 (ATG5), microtubule-associated protein 1 light chain 3 Ⅱ(LC3 Ⅱ) mRNA and TNF-α, caspase-3, LC3 Ⅱ, p62 proteins were detected. Results Compared with the control group, the mRNA expression levels of IL-6, TNF-α, TGF-β, ATG5, and LC3 Ⅱ in LPS group were significantly increased, and the differences were statistically significant (P ＜ 0.05). Compared with the control group, the protein expression levels of TNF-α, LC3 Ⅱ, p62 and caspase-3 in LPS group were increased, and the differences were statistically significant (P ＜ 0.05). The mRNA expression levels of IL-6, TNF-α, TGF-β, ATG5, and LC3 Ⅱ in Kujin Tablets group were significantly lower than those in LPS group, the differences were statistically significant (P ＜ 0.05). The protein expression levels of TNF-α, LC3 Ⅱ, p62, and caspase-3 in Kujin Tablets group were significantly lower than those in LPS group, and the differences were statistically significant (P ＜ 0.05). Conclusion Kujin Tablets can significantly reduce LPS-induced cell inflammation, reduce apoptosis and improve cell viability. Autophagy is involved in the anti-inflammatory process of Kujin Tablets.

Key words： Kujin Tablets Lipopolysaccharide Macrophages Inflammation Autophagy

基金资助:江苏省卫生健康委员会科研课题面上项目（H2017013）。

通讯作者: ▲通讯作者

引用本文:

刘晴朱春雪刘承雨王一成何美娟何嫣婕黄汉鹏▲. 苦金片对脂多糖诱导巨噬细胞炎症反应的影响[J]. 中国医药导报, 2022, 19(2): 21-24.
LIU Qing ZHU Chunxue LIU Chengyu WANG Yicheng HE Meijuan HE Yanjie HUANG Hanpeng▲. Effect of Kujin Tablets on inflammatory response of macrophages induced by lipopolysaccharide#br#. 中国医药导报, 2022, 19(2): 21-24.

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[1] Pajares M，I Rojo A，Manda G，et al. Inflammation in Parkinson’s Disease：Mechanisms and Therapeutic Implications [J]. Cells，2020，9（7）：1687.
[2] Boeddinghaus R，Whyte A. The many faces of periapical inflammation [J]. Clin Radiol，2020，75（9）：675-687.
[3] Mahtta D，Sudhakar D，Koneru S，et al. Targeting Inflammation After Myocardial Infarction [J]. Curr Cardiol Rep，2020，22（10）：110.
[4] Roe K. An inflammation classification system using cytokine parameters [J]. Scand J Immunol，2021，93（2）：e12970.
[5] Zahid MDK，Rogowski M，Ponce C，et al. CCAAT/enhancer-binding protein beta（C/EBPβ）knockdown reduces inflammation，ER stress，and apoptosis，and promotes autophagy in oxLDL-treated RAW264.7 macrophage cells [J]. Mol Cell Biochem，2020，463（1/2）：211-223.
[6] Mizushima N，Levine B. Autophagy in Human Diseases [J]. N Engl J Med，2020，383（16）：1564-1576.
[7] Painter JD，Galle-Treger L，Akbari O. Role of Autophagy in Lung Inflammation [J]. Front Immunol，2020，11：1337.
[8] Di Muzio F，Barucco M，Guerriero F. Diagnosis and treatment of acute pharyngitis/tonsillitis：a preliminary observational study in General Medicine [J]. Eur Rev Med Pharmacol Sci，2016，20（23）：4950-4954.
[9] Haddad JD，Ng P，James T. Empiric Treatment for Acute Pharyngitis [J]. Am Fam Physician，2019，100（11）：713-714.
[10] 易新，林李辉.金叶败毒颗粒佐治急性咽炎临床观察[J].中国药业，2021，30（2）：65-67.
[11] 王洪玉.黄芩茎叶解毒胶囊联合阿奇霉素治疗急性咽炎的临床研究[J].现代药物与临床，2020，35（9）：1878-1881.
[12] 王殿一，李岩，张竞飞，等.牛黄利咽丸治疗急喉痹的临床观察[J].中医药学报，2020，48（6）：38-40.
[13] 黄普威.针刺放血疗法配合咽超声波雾化吸入法治疗急喉痹风热外袭证的疗效观察[D].长春：长春中医药大学，2020.
[14] 李木子，张浩，朱粉霞.苦地丁的化学成分研究[J].华西药学杂志，2019，34（3）：218-224.
[15] 李木子，张浩，崔国倩，等.苦地丁的抗炎药效物质基础组分及作用机制研究[J].中国中药杂志2020，45（11）：2586-2594.
[16] 刘玉峰，李鲁盼，马海燕，等.金银花化学成分及药理作用的研究进展[J].辽宁大学学报：自然科学版，2018，45（3）：255-262.
[17] Li Y，Li W，Fu C，et al. Lonicerae japonicae flos and Lonicerae flos：a systematic review of ethnopharmacology，phytochemistry and pharmacology [J]. Phytochem Rev，2019：1-61.
[18] Liao H，Ye J，Gao L，et al. The main bioactive compounds of Scutellaria baicalensis Georgi. for alleviation of inflammatory cytokines：A comprehensive review [J]. Biomed Pharmacother，2021，133：110917.
[19] Jin BR，Chung KS，Kim HJ，et al. Chinese Skullcap（Scutellaria baicalensis Georgi）inhibits inflammation and proliferation on benign prostatic hyperplasia in rats [J]. J Ethnopharmacol，2019，235：481-488.
[20] Kee JY，Hong SH. Inhibition of Mast Cell-Mediated Allergic Responses by Arctii Fructus Extracts and Its Main Compound Arctigenin [J]. J Agric Food Chem，2017，65（43）：9443-9452.
[21] Zhong Y，Lee K，Deng Y，et al. Arctigenin attenuates diabetic kidney disease through the activation of PP2A in podocytes [J]. Nat Commun，2019，10（1）：4523.
[22] Sheu SY，Hong YW，Sun JS，et al. Radix Scrophulariae extracts（harpagoside）suppresses hypoxia-induced microglial activation and neurotoxicity [J]. BMC Complement Altern Med，2015，15：324.
[23] Deretic V，Levine B. Autophagy balances inflammation in innate immunity [J]. Autophagy，2018，14（2）：243-251.
[24] Deretic V，Klionsky DJ. Autophagy and inflammation：A special review issue [J]. Autophagy，2018，14（2）：179-180.
[25] Wang XX，Zhang B，Xia R，et al. Inflammation，apoptosis and autophagy as critical players in vascular dementia [J]. Eur Rev Med Pharmacol Sci，2020，24（18）：9601-9614.