Abstract：Objective To investigate the effect of Dihydrotestosterone (DHT) on prostatic stromal cell inflammation in aged benign prostatic hyperplasia (BPH) patients. Methods A total of 20 patients who underwent transurethral resection prostate in the Department of Urology, Affiliated Hospital of Inner Mongolia Medical University from January 2016 to May 2017 were collected. Primary prostatic stromal cells were cultured by tissue block method. The expression of Cytokeratin, Desmin, Vimentin and α -smooth muscle actin (α-SMA) in cultured stromal cells of the third generation were determined by immunohistochemical staining. The third generation of cells were cultured in the same size petri dishes and named as blank group, control group and experimental group. Culture medium with the same concentration and dose was added to the blank group. The control group and experimental group were added with tumor necrosis factor-α (TNF-α) 10 ng/ml, 30 nmol/L DHT was added in the experimental group 5 h later. Then co-culture for 24 h, take 100 μl supernatant from three groups, with 4 rewells in each group. The concentrations of interleukin (IL)-6, IL-8, monocyte chemotactic protein-1 (MCP-1) and basic fibroblast growth factor (bFGF) in each group were detected by enzyme-linked immunosorbent assay. Results Primary prostatic interstitial cells were cultured in vitro. Cytokeratin and Desmin were negatively expressed in the third generation of prostatic interbasal cells. Vimentin and α-SMA were positive. The concentrations of IL-6, IL-8, MCP-1, and bFGF in the control group were higher than those in the blank group, the differences were statistically significant (P ＜ 0.05), while the concentrations of IL-6, IL-8, MCP-1 and bFGF in the experimental group were lower than those in the control group, the differences were statistically significant (P ＜ 0.05). Conclusion DHT has a direct anti-inflammatory effect on prostatic interstitial cells in aged patients with prostatic hyperplasia.
[1] Taoka R，Kakehi Y. The influence of asymptomatic inflammatory prostatitis on the onset and progression of lower urinary tract symptoms in men with histologic benign prostatic hyperplasia [J]. Asian J Urol，2017，4（3）：158-163.
[2] Quintar AA，Maldonado CA. Androgen regulation of host defenses and response to inflammatory stimuli in the prostate gland [J]. Cell Biol Int，2017,41（11）：1223-1233.
[3] Gandaglia G，Zaffuto E，Fossati N，et al. The role of prostatic inflammation in the development and progression of benign and malignant diseases [J]. Curr Opin Urol，2017，27（2）：99-106.
[4] 白安胜，汪峰，贾军琪，等.前列腺增生症患者合并前列腺炎对血清PSA的影响[J].现代泌尿外科杂志，2012，17（2）：177-179.
[5] Huo YW，DingY，Zhu GN，et al. Primary Culture Method of Prostate Stromal Cells [J]. Letters in Biotechnology，2019.
[6] 陈君秀.常用良性前列腺增生细胞系及动物模型研究进展[J].中华实验外科杂志，2020，37（8）：1555-1559.
[7] Liu LR，Qiang W. Primary culture of benign prostatic hyperplasia stromal cell [J]. Sichuan Da Xue Xue Bao，2010， 41（3）：518-522.
[8] Vignozzi L，Cellai I，Santi R，et al. Antiinflammatory effect of androgen receptor activation in human benign prostatic hyperplasia cells [J]. J Endocrinol，2012，214（1）：31.
[9] Zlotta AR，Egawa S，Pushkar D，et al. Prevalence of inflammation and benign prostatic hyperplasia on autopsy in Asian and Caucasian men [J]. Eur Urol，2014，13（1）：619-622.
[10] Ren X，Chunlei WU，Qinnan YU，et al. Correlation of IL-8 and IL-6 in prostatic fluid with serum prostate-specific antigen level in patients with benign prostatic hyperplasia complicated by prostatitis [J]. Nan Fang Yi Ke Da Xue Xue Bao，2016，36（1）：135-139.
[11] Gross NT，Wang J，Smith G，et al. Abstract 1015：Molecular dissection of androgen receptor signaling in prostatic endothelial，epithelial，and stromal cells[C]//Proceedings：AACR Annual Meeting 2019;March 29-April 3，2019， Atlanta，GA，2019.
[12] Zubkov AY，Antonov NA. Chronic bacterial prostatitis associated with androgen deficiency [J]. Kazanskiǐ meditsinskiǐ zhurnal，2020，101（3）：389-393.
[13] Vignozzi L，Gacci M，Cellai I，et al. Fat boosts，while androgen receptor activation counteracts，BPH-associated prostate inflammation [J]. Prostate，2013，73（8）：789-800.
[14] Wu Y，Pan H，Wang WM，et al. A possible relationship between serum sex hormones and benign prostatic hyperplasia/lower urinary tract symptoms in men who underwent transurethral prostate resection [J]. Asian J Androl，2017，19（2）：230-233.
[15] 张志超，彭靖.睾酮补充治疗与改善下尿路症状的研究进展[J].中华男科学杂志，2020，26（5）：387-391.
[16] 刘念，刘向云.AR信号通路在良性前列腺增生中的研究进展[J].中国细胞生物学学报，2020，42（9）：1669-1675.
[17] Jia YL，Liu X，Yan JY，et al. The alteration of inflammatory markers and apoptosis on chronic prostatitis induced by estrogen and androgen [J]. Int Urol Nephrol，2015，47（1）：39.
[18] Rev A，Oef A，Dcm B，et al. The role of the androgen receptor in prostate development and benign prostatic hyperplasia：A review [J]. Asian J Urol，2020，7（3）：191-202.
[19] Welsh M，Moffat L，Alan MN，et al. Smooth muscle cell-specific knockout of androgen receptor： a new model for prostatic disease [J]. Endocrinology，2011，152（9）：3541-3551.
[20] 黄林.针刺尿三针对良性前列腺增生大鼠的疗效[J].中国老年学杂志，2020，40（10）：2196-2200.
[21] Karan D，Dubey S. From Inflammation to Prostate Cancer：The Role of Inflamma-Somes [J]. Advances in Urology，2016，2016（1）：1-5.
[22] Saad F. The emancipation of testosterone from niche hormone to multi-system player [J]. Asian J Androl，2015， 17（1）：58-60.
[23] Cui Q，Han L，Cheng J，et al. Relevance of levels of immunoglobulins，cytokines，Chemokines in the prostatic secretion to chronic prostatitis [J]. Current Immunology，2017.
[24] 王波，罗光恒，王振，等.不同比例浓度雌雄激素诱导下大鼠前列腺炎症反应模型的建立[J].四川大学学报，2021，52（3）：477.
京公网安备 11010502046598号