Effects of Chinese medicine barbary wolfberry fruit and danshen root for tonifying deficiency and promoting blood circulation on the expression of TNF-α and IL-1β in the retina of rd10 mice#br#
JIANG Pengfei1,2 YAO Zhen1,2▲ OU Chen1,2 YANG Yijing1,2 PENG Jun1,3 XU Jian4 PENG Qinghua1,2▲
1.College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Hunan Province, Changsha 410208, China;
2.Hunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese Medicine, Hunan Province, Changsha 410208, China;
3.Department of Ophthalmology, the First Hospital of Hunan University of Chinese Medicine, Hunan Province, Changsha 410007, China;
4.Department of Ophthalmology, Shanghai Oriental Hospital, Shanghai 200120, China
Abstract：Objective To observe the effects of Chinese medicine barbary wolfberry fruit and danshen root for tonifying deficiency and promoting blood circulation on the expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the retina of rd10 mice. Methods Forty four-week-old rd10 (Pde6b, -/-) mice with the body weight of 18-20 g were divided into model group, control group, barbary wolfberry fruit group, danshen root group, and barbary wolfberry fruit with danshen root group according to random number table method, with eight mice in each group. Physiological saline, vitamin A aqueous solution, Barbary Wolfberry Fruit Chinese medicine Formula Granules aqueous solution, Danshen Root Chinese medicine Formula Granules aqueous solution, Barbary Wolfberry Fruit and Danshen Root Chinese medicine Formula Granules aqueous solution were given 20 ml/(kg·d) by gavage, respectively. Eight C57 wild-type (Pde6b, +/+) mice were given the same amount of normal saline by gavage. The samples were taken after 28 days of gavage. The expression levels of TNF-α and IL-1β in the retina of mice in each group were detected by real-time fluorescence quantitative PCR and Western blot. Results Compared with blank group, the protein and mRNA relative expression levels of TNF-α and IL-1β in model group were increased (P ＜ 0.05). Compared with model group, the protein and mRNA relative expression levels of TNF-α and IL-1β in barbary wolfberry fruit group, danshen root group, and barbary wolfberry fruit with danshen root group were decreased (P ＜ 0.05). Compared with barbary wolfberry fruit group, the relative expression levels of TNF-α and IL-1β protein and IL-1β mRNA in barbary wolfberry fruit with danshen root group were decreased (P ＜ 0.05). Compared with danshen root group, the protein and mRNA relative expression levels of TNF-α and IL-1β in barbary wolfberry fruit with danshen root group were decreased (P ＜ 0.05). Conclusion Chinese medicine for tonifying deficiency and promoting blood circulation, Chinese medicine barbary wolfberry fruit and danshen root can reduce the expression levels of TNF-α and IL-1β in the retina of rd10 mice, and protect the ultrastructure of the retina.
蒋鹏飞1,2 姚震1,2▲ 欧晨1,2 杨毅敬1,2 彭俊1,3 徐剑4 彭清华1,2▲. 补虚活血中药枸杞子丹参对rd10小鼠视网膜TNF-α、IL-1β表达的影响[J]. 中国医药导报, 2021, 18(35): 15-18,27.
JIANG Pengfei1,2 YAO Zhen1,2▲ OU Chen1,2 YANG Yijing1,2 PENG Jun1,3 XU Jian4 PENG Qinghua1,2▲. Effects of Chinese medicine barbary wolfberry fruit and danshen root for tonifying deficiency and promoting blood circulation on the expression of TNF-α and IL-1β in the retina of rd10 mice#br#. 中国医药导报, 2021, 18(35): 15-18,27.
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