Effect of Ziyin Tongluo Decoction on bone metabolism and bone mineral density in ovariectomized osteoporosis rats#br#
SUN Kai1 CHEN Rongbin2 YANG Yingdong1 LI Yong2
1.The Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510405, China;
2.the Third Branch of Department of Orthopedics, Zhuhai Hospital, Traditional Chinese Medical Hospital of Guangdong Province, Guangdong Province, Zhuhai 519000, China
Abstract：Objective To investigate the effect of Ziyin Tongluo Decoction on bone metabolism and bone mineral density in ovariectomized osteoporosis rats. Methods Fifty SD female rats were selected to establish ovariectomized rat model, and another ten SD rats were selected as the sham operation group. The rats were divided into model group, Western medicine group (Alendronate Sodium [10.51 mg/kg] and Alfacalccitalol [0.075 μg/kg]), Ziyin Tongluo Decoction low-dose (1.34 g/kg), medium-dose (2.68 g/kg) and high-dose (4.02 g/kg) groups according to random number table method, with ten rats in each group. They were treated by gavage. After 12 weeks of treatment, bone mineral density(BMD), bone microstructure and serum contents of procollagen Ⅰ N-terminal propeptide (PⅠNP), N-terminal telopeptide of type Ⅰ collagen(NTX)， and C-telopeptide of type Ⅰ collagen (CTX-Ⅰ) were determined. Results Compared with sham operation group, BMD, bone volume fraction, bone trabecular number, bone trabecular thickness, and PⅠNP level were decreased in model group, and the differences were statistically significant (P ＜ 0.05). The trabecular separation, trabecular pattern factor, structural model index, NTX, and CTX-Ⅰ levels were increased in model group, and the differences were statistically significant (P ＜ 0.05). Compared with model group, BMD, bone volume fraction, bone trabecular number, bone trabecular thickness and PⅠNP level in Ziyin Tongluo Decoction groups were increased; the trabecular separation, trabecular pattern factor, structural model index, NTX, and CTX-Ⅰ levels were decreased, and the differences were statistically significant (P ＜ 0.05). There was no significant difference between groups with different doses of Ziyin Tongluo Decoction (P > 0.05). Conclusion Ziyin Tongluo Decoction can improve bone metabolism, increase bone density and improve bone microstructure of ovariectomized rats, and achieve the effect of postmenopausal osteoporosis treatment.
孙凯1 陈荣彬2 杨英东1 李勇2. 滋阴通络方对去卵巢骨质疏松大鼠骨代谢及骨密度的影响[J]. 中国医药导报, 2021, 18(34): 26-29,38.
SUN Kai1 CHEN Rongbin2 YANG Yingdong1 LI Yong2. Effect of Ziyin Tongluo Decoction on bone metabolism and bone mineral density in ovariectomized osteoporosis rats#br#. 中国医药导报, 2021, 18(34): 26-29,38.
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