Therapeutic effect of Shenyuan Yiqi Huoxue Capsules on cerebral thrombosis induced by Punatinib in zebrafish#br#
QIN Xiaomei1 GUO Shuaijie1 LI Yue1 LI Sinai1 LIU Weihong1 WANG Xuyun2 LIU Hongxu3 ZHOU Mingxue1▲
1.Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China; 2.Andrology of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China;
3.Department of Cardiovascular, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
Abstract：Objective To study the therapeutic effect of Shenyuan Yiqi Huoxue Capsules (hereinafte referred to as “Shenyuandan Capsules”) on cerebral thrombosis induced by Punatinib in zebrafish. Methods A total of 720 zebrfish were divided into four batches， 180 zebrfish were selected from each batch and placed in a six-well plate with 30 fish in each well. They were divided into six groups: normal control group, model group, positive control group and Shenyuandan Capsules low, middle, and high dose group, which were respectively given fish culture water, Prunatinib, Prunatinib and water-dissolved Aspirin (50 μg/ml) or 55.6, 167.0, 500.0 μg/ml Shenyuandan Capsules. After treatment for 18 h, the number of zebrafish forming cerebral thrombosis was observed; the kinematic velocity of zebrafish was measured by a behavioral analyzer; the blood flow video of zebrafish was recorded by the blood heartbeat analysis software to analyze the blood flow velocity of zebrafish; apoptosis number of brain cells in zebrafish was analyzed by acridine orange staining. Results Compared with model group, the incidence of cerebral thrombosis of zebrafish in Shenyuandan Capsules low, middle, and high dose groups were significantly decreased, and the differences were highly statistically significant (P < 0.01); the kinematic velocity of zebrafish in Shenyuandan Capsules low, middle, and high groups were significantly increased, and the differences were statistically significant (P < 0.01); blood flow velocity of zebrafish in Shenyuandan Capsules medium and high dose groups were significantly increased, and the differences were highly statistically significant (P < 0.01); apoptosis number of zebrafish brain cells in Shenyuandan Capsules high dose group were significantly decreased, and the difference was highly statistically significant (P < 0.01). Conclusion Shenyuandan Capsules can significantly improve the incidence of cerebral thrombosis and kinematic velocity induced by Pratinib in zebrafish, and the mechanism may be related to inhibiting brain cell apoptosis in zebrafish.
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